The objective of the diploma paper "Organizational Behaviour and Corporate Culture" is to evaluate the current level of the organizational behaviour and the corporate culture in the allowance organization Centrum služeb pro silniční dopravu, that is managed by the Traffic Department of the Czech Republic. The partial aims are finding imperfections by means of questionnaires and the draft of measures, which would improve the present condition. The theoretical part refers to the account of conceptions of the organizational behaviour and the corporate culture. In the practical part the organization Centrum služeb pro silniční dopravu is described. This part is focused on activities, that have in light of organizational behaviour and corporate culture connection with the aims of this diploma paper. Furthermore in this part there are charactered results from the questionnaire research.