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RecurrentFBN1Mutation (R62C) in a Chinese Family With Isolated Ectopia Lentis

Authors
Journal
American Journal of Ophthalmology
0002-9394
Publisher
Elsevier
Publication Date
Volume
141
Issue
6
Identifiers
DOI: 10.1016/j.ajo.2005.12.044
Disciplines
  • Biology

Abstract

Purpose To examine the fibrillin-1 ( FBN1) gene for mutations in members of a Chinese family with isolated ectopia lentis. Design Clinically relevant laboratory investigation. Methods Family members underwent clinical examinations. Genomic DNA was extracted from leukocytes of peripheral blood from the available members and 100 controls for mutation analysis. The 65 exons of FBN1 were amplified by polymerase chain reaction and screened for mutations by a combination of denaturing high-performance liquid chromatography analysis and direct DNA sequencing. Results A mutation, c.184C→T in exon 2 of FBN1, which results in substitution of arginine by cysteine at position 62 of the fibrillin-1 protein (p.R62C) in all affected family members but in none of the unaffected individuals. Conclusions A recurrent mutation of FBN1 gene resulted in an arginine-to-cysteine residue (p.R62C), is responsible for the patients with isolated ectopia lentis in a Chinese family.

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