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A rapid chromatographic method for the production of preparative amounts of xylulose

Authors
Journal
Enzyme and Microbial Technology
0141-0229
Publisher
Elsevier
Publication Date
Volume
16
Issue
5
Identifiers
DOI: 10.1016/0141-0229(94)90153-8
Keywords
  • D-Xylulose
  • Enzymatic Isomerization
  • Precipitation
  • Ion-Exchange Chromatography

Abstract

Abstract High-purity d-xylulose > 97% was prepared from d-xylose in gram quantities using a simple three-step procedure; enzymatic isomerization with commercial glucose isomerase (xylose isomerase EC. 5.3.1.5), ethanol precipitation, and ion-exchange chromatography on an anionic resin in sulfite form. In the chromatographic step, 0.7–1.4 g xylulose was produced within 10–15 h. Impurities in the preparation are of sugar origin and may consist of glucose, ribulose, and xylose. Fractions of high purity, > 99%, but in small amounts, could be produced in the chromatographic step. When the procedure was simplified by performing the precipitation in a saturated isomerization solution, almost identical results were obtained. If the precipitation step was excluded, it resulted in poorer separation in the chromatographic step, but xylulose could still be produced with a purity with a purity of 96–97%, though in smaller amounts, 0.35–0.74g.

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