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A fluorometric procedure for determination of β-glucosidase

Authors
Journal
Phytochemistry
0031-9422
Publisher
Elsevier
Publication Date
Volume
11
Issue
6
Identifiers
DOI: 10.1016/s0031-9422(00)90157-2
Disciplines
  • Biology

Abstract

Abstract A rapid fluorometric technique has been developed for the measurement of β-glucosidase activity. The change in concentration of a non-fluorescent substrate such as 4-methylumbelliferone β- d-glucoside is measured directly by recording increases in fluorescence as the aglycone is produced. Sweet clover, corn leaf and almond emulsin were used as enzyme sources in the development of the method which resulted in good linearity in Lineweaver-Burk determinations.

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