Affordable Access

Publisher Website

Proteomics in cancer cell research: an analysis of therapy resistance

Authors
Journal
Pathology - Research and Practice
0344-0338
Publisher
Elsevier
Publication Date
Volume
200
Issue
2
Identifiers
DOI: 10.1016/j.prp.2004.02.003
Keywords
  • 2D-Page
  • Cancer
  • Drug Resistance
  • Thermoresistance
Disciplines
  • Biology

Abstract

Abstract Proteomics, the global analysis of expressed cellular proteins, provides powerful tools for the detailed comparison of proteins from normal and neoplastic tissue. In particular, cancer cell culture models are suited for applying proteomics techniques, such as two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry, to identify specific protein expression profiles and/or proteins that may be associated with a defined phenotype of the cancer cells. As an instance of such an application of proteomics techniques, the detailed proteome analyses of different drug-resistant and thermoresistant cancer cell lines will be discussed. Finally, the potential roles of newly identified factors in a distinct biological mechanism have to be proven by functional studies. This experimental validation strategy will be discussed for two different factors identified by 2D-PAGE analyses of drug-resistant carcinoma cell lines, the “transporter associated with antigen presentation 1” (TAP1) and 14-3-3 σ.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Proteomics in cancer cell research: an analysis of...

on Pathology - Research and Pract... 2004

Study of therapy resistance in cancer cells with f...

on Clinical chemistry and laborat... March 2002

Proteomics in cancer research.

on International Journal of Gynec... 2002
More articles like this..