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Separation and characterisation of glycoproteins from normal, pregnancy, and acute inflammatory sera

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
397
Identifiers
DOI: 10.1016/s0021-9673(01)85004-4
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

Abstract A system of multiple chromatography combining anion-exchange, gel filtration, and affinity chromatography has been devised to separate several acute phase proteins from serum, including α 1 acid glycoprotein, transferrin, α 1-antichymotrypsin, α 1-antitrypsin and haemopexin. Only 5–15 ml of blood is required to provide milligram quantities of the purified proteins for further biochemical analysis. The system has been applied in a study of pregnancy and some diseases in order to investigate the changes in the serum protein profile and in individual proteins.

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