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Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro

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Abstract ral Reproductive Biology and ss BioMed CentEndocrinology Open AcceResearch Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro Donal J Sexton, Michael W O'Reilly, Anne M Friel and John J Morrison* Address: Department of Obstetrics and Gynaecology, National University of Ireland Galway, Clinical Science Institute, University College Hospital, Newcastle Road, Galway, Ireland Email: Donal J Sexton - [email protected]; Michael W O'Reilly - [email protected]; Anne M Friel - [email protected]; John J Morrison* - [email protected] * Corresponding author Abstract Background: 17alpha-hydroxyprogesterone caproate (17P) administration reportedly improves outcome for women with a previous spontaneous preterm delivery. This study, using in vitro strips of human uterine smooth muscle, aimed to investigate the direct non-genomic effects of 17P on spontaneous and induced contractions in tissues obtained during pregnancy, and in the non- pregnant state. Methods: Biopsies of human myometrium were obtained at elective cesarean section, and from hysterectomy specimens, and dissected strips suspended for isometric recordings. The effects of 17P (1 nmol/L -10 micro mol/L) on spontaneous and agonist-induced (oxytocin 0.5 nmol/L for pregnant, phenylephrine 10 µmol/L for non-pregnant) contractions were measured. Integrals of contractile activity, including the mean maximal inhibition values (MMI) observed at the maximal concentration, were compared with those from simultaneously run control strips. Results: There was no significant direct effect exerted by 17P on pregnant or non-pregnant human myometrial contractility. The MMI ± SEM for spontaneous contractions in pregnant myometrium was 4.9% ± 7.2 (n = 6; P = 0.309) and for oxytocin-induced contractions was 2.2% ± 1.3 (n = 6; P = 0.128). For non-pregnant myometrium, the MMI ± SEM for spontaneous contractions was 8.8% ± 11.0 (n = 6;

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