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The protein and nucleic acid of beet yellows virus

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
77
Issue
1
Identifiers
DOI: 10.1016/0042-6822(77)90410-x
Disciplines
  • Biology

Abstract

Abstract By analysis on SDS-polyacrylamide gels, the coat protein of beet yellows virus was shown to consist of a single polypeptide of molecular weight about 22,400. The amino acid composition and tryptic peptide map also indicate a molecular size close to 22,000. Viral RNA comprising 6% by weight of the particles is single stranded and has the base composition U, 23%; C, 22%; A, 27%; G, 28%. Its size, estimated by sedimentation and gel electrophoresis, is close to 4.6 × 10 6. We propose that in the virus particle the single helical RNA strand is embedded at a radius of 3.3 nm in a protein coat comprised of approximately 3400 protein subunits, giving a total particle weight of 80 million daltons.

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