Abstract Adenosine, a modulator of pain processing in the spinal cord, is metabolized by adenosine kinase and adenosine deaminase. In this study we determined which of these mechanisms is more important for the regulation of endogenous adenosine levels in the rat spinal cord. The effects of the adenosine kinase inhibitors, 5′-deoxyadenosine (NH 2dAD) and iodotubercidin (IOT), and the adenosine deaminase inhibitor, 2′-deoxycoformycin (DCF), on adenosine release in a spinal cord superfusion model were studied. DCF markedly increased basal adenosine levels detected in perfusates and was more potent than NH 2dAD and IOT in this regard. Coadministration of DCF with NH 2dAD produced an enhanced effect compared to the inhibitors alone. NH 2dAD, but not DCF, potentiated morphine-evoked adenosine release. These results suggest that adenosine deaminase may be the predominant pathway for adenosine metabolism in this experimental model.