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Self-assembly of avidin and streptavidin with multifunctional biotin molecules

Authors
Journal
Thin Solid Films
0040-6090
Publisher
Elsevier
Publication Date
Volume
244
Identifiers
DOI: 10.1016/0040-6090(94)90572-x
Disciplines
  • Biology

Abstract

Abstract We report the synthesis of a water-soluble tetrafunctional biotin ligand based on the porphyrin moiety and its behaviour at the air-water interface. The addition of streptavidin or avidin to the subphase is shown to cause a significant expansion of the isotherm of the tetrabiotinylated ligand, indicating a strong interaction between the protein and the ligand. The addition of inactive protein to the subphase caused no such change from which it is deduced that non-specific interactions could not have been responsible for the effects observed with the active proteins. Supplementary experiments using column chromatography provide evidence for the formation of a high molecular weight polymer when the tetrabiotinylated ligand and active protein are mixed.

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