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Plasmolysis of precultured immature embryos improvesAgrobacteriummediated gene transfer to rice (Oryza sativaL.)

Authors
Journal
Plant Science
0168-9452
Publisher
Elsevier
Publication Date
Volume
130
Issue
1
Identifiers
DOI: 10.1016/s0168-9452(97)00211-2
Keywords
  • Gene Transfer
  • Rice
  • Agrobacterium
  • Plasmolysis

Abstract

Abstract Efficiency of Agrobacterium mediated gene transfer was improved in rice by microprojectile pretreatment. Fertile transgenic plants were easily recovered using this protocol. Since an osmotic treatment is part of the bombardment protocol, we studied the effect of plasmolysis alone (i.e. without any microprojectiles) on transient expression of the gus gene transferred by Agrobacterium to precultured rice embryos. We report here for the first time that plasmolysis alone as a single pretreatment, yielded an even higher number of cells expressing the marker gene than the combination of microprojectile bombardment and osmotic treatment. This indicates that the effect of the osmoticum on gene transfer is independent from bombardment.

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