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The effect of CpG-ODN on antigen presenting cells of the foal

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BioMed Central
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PMC
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  • Original Research

Abstract

1476-8518-5-1.fm ral Journal of Immune Based Therapies ss BioMed Centand Vaccines Open AcceOriginal research The effect of CpG-ODN on antigen presenting cells of the foal M Julia BF Flaminio*1, Alexandre S Borges2, Daryl V Nydam3, David W Horohov4, Rolf Hecker5 and Mary Beth Matychak1 Address: 1Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA, 2Departamento de Clinica Veterinaria, Faculdade de Medicina Veterinaria e Zootecnia, Universidade Estadual Paulista 'Julio de Mesquita Filho', UNESP-Campus de Botucatu, SP, Brazil, 3Department of Population Medicine and Diagnostics Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA, 4Department of Veterinary Science, Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY, USA and 5Qiagen GmbH, Hilden, Germany; current address Tübingen, Germany Email: M Julia BF Flaminio* - [email protected]; Alexandre S Borges - [email protected]; Daryl V Nydam - [email protected]; David W Horohov - [email protected]; Rolf Hecker - [email protected]; Mary Beth Matychak - [email protected] * Corresponding author Abstract Background: Cytosine-phosphate-guanosine oligodeoxynucleotide (CpG-ODN) has been used successfully to induce immune responses against viral and intracellular organisms in mammals. The main objective of this study was to test the effect of CpG-ODN on antigen presenting cells of young foals. Methods: Peripheral blood monocytes of foals (n = 7) were isolated in the first day of life and monthly thereafter up to 3 months of life. Adult horse (n = 7) monocytes were isolated and tested once for comparison. Isolated monocytes were stimulated with IL-4 and GM-CSF (to obtain dendritic cells, DC) or not stimulated (to obtain macrophages). Macrophages and DCs were stimulated for 14–16 hours with either CpG-ODN, LPS or not stimulated. The stimulated and non-stimulated cells were tested for cell surface markers (CD86 and MHC cl

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