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A Simple Way to Measure Ethanol Sensitivity in Flies

Authors
Journal
Journal of Visualized Experiments
1940-087X
Publisher
MyJoVE Corporation
Publication Date
Issue
48
Identifiers
DOI: 10.3791/2541
Source
Legacy
Keywords
  • Neuroscience
Disciplines
  • Medicine

Abstract

Low doses of ethanol cause flies to become hyperactive, while high doses are sedating. The sensitivity to ethanol-induced sedation of a given fly strain is correlated with that strain s ethanol preference, and therefore sedation is a highly relevant measure to study the genetics of alcohol responses and drinking. We demonstrate a simple way to expose flies to ethanol and measure its intoxicating effects. The assay we describe can determine acute sensitivity, as well as ethanol tolerance induced by repeat exposure. It does not require a technically involved setup, and can therefore be applied in any laboratory with basic fly culture tools.

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