Affordable Access

Publisher Website

Combined monoclonal antibody typing, multilocus enzyme electrophoresis, soluble protein profiles and plasmid analysis of clinical and environmentalLegionella pneumophilaserogroup 1 isolated in a Portuguese hospital

Authors
Journal
Journal of Hospital Infection
0195-6701
Publisher
Elsevier
Publication Date
Volume
30
Issue
2
Identifiers
DOI: 10.1016/0195-6701(95)90150-7
Keywords
  • Legionella Pneumophila
  • Nosocomial Infection
  • Bacterial Typing Methods
  • Clinical And Environmental Specimens
Disciplines
  • Biology
  • Ecology
  • Geography
  • Medicine

Abstract

Abstract Sixteen strains of Legionella pneumophila serogroup 1 isolated from patients and the environment at Santa Cruz Hospital were examined using four typing methods. Monoclonal antibodies showed that all the isolates belonged to subgroup Pontiac and mainly to subtype Allentown. With multilocus enzyme electrophoresis nine subtypes (ETs) were revealed; each strain had the same profile with electrophoresis of soluble proteins, and plasmid analysis revealed that only two of the strains studied contained plasmids. Among these methods, multilocus enzyme electrophoresis was the most discriminative as a single epidemiological marker. Problems concerning the microbiological examination of environmental specimens and correlation with clinical strains confirmed the difficulty of investigating an outbreak source of legionellosis and the need for careful evaluation of the results.

There are no comments yet on this publication. Be the first to share your thoughts.