Affordable Access

Publisher Website

Purification by centrifugal partition chromatography of amphiphilic compounds, glycolipids and pseudo-glycolipids synthesized by using cells

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
1178
Identifiers
DOI: 10.1016/j.chroma.2007.11.094
Keywords
  • Glycolipids
  • Neutral Lipid
  • Acidic Lipid
  • Ganglioside
  • Centrifugal Partition Chromatograph

Abstract

Abstract Centrifugal partition chromatography (CPC) was applied to separate amphiphilic glycolipids and pseudo-glycolipids synthesized by using cells. Neutral and acidic lipid fractions were isolated by CPC under suitable conditions respectively. Separation of neutral lipid, Gb3-type and Gb4-type oligosaccharide synthesized by using cells, was performed with a two-phase solvent system composed of chloroform–methanol–water at a volume ratio of 5:6:4. On the other hand, separation of acidic lipid, GM3-type oligosaccharide synthesized by using cells, and ganglioside extracted from rat brain were performed with a two-phase solvent system composed of butanol–ethanol–1% acetic acid at a volume ratio of 4:1:5. 8.3 mg of Gb3 analogue, 5.1 mg of Gb4 analogue, and 19.5 mg of GM3 analogue were purified from 3.2 l of culture medium obtained by incubation of African green-monkey kidney (Vero) cells with 50 μM n-dodecyl β-lactoside using CPC.

There are no comments yet on this publication. Be the first to share your thoughts.