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GKLF in thymus epithelium as a developmentally regulated element of thymocyte-stroma cross-talk

Authors
Journal
Mechanisms of Development
0925-4773
Publisher
Elsevier
Publication Date
Volume
81
Identifiers
DOI: 10.1016/s0925-4773(98)00237-8
Keywords
  • Thymus
  • Stromal Cells
  • Gene Regulation
  • Transcription Factors
  • Hematopoiesis

Abstract

Abstract Gut-enriched Krüppel-like factor (GKLF) is a transcriptional regulator expressed in differentiated epithelia. We identified GKLF transcript as a regulated element in thymic epithelium of recombinase-deficient mice during thymus development induced by anti-CD3 antibody injection. This treatment recapitulates the organogenetic process depending on productive rearrangement of T cell receptor (TCR) β gene with thymocytes expansion and acquisition of the CD4 +8 + double positive phenotype. In wildtype mice, GKLF is expressed very early in embryogenesis and becomes intensely up-regulated in thymus epithelium at day 18 of gestation when TCR β expressing cells have selectively expanded and express both CD4 and CD8. The results presented here suggest that thymocytes may regulate GKLF transcriptionally in the cortical epithelium at the developmental check-point controlled by TCR β gene rearrangement. Furthermore, GKLF expression in hematopoietic stroma might suggest the thus far uncharacterised participation of this factor in hematopoiesis.

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