Affordable Access

Publisher Website

ARMS test for diagnosis of factor VLeidenmutation and allele frequencies in France

Authors
Journal
Molecular and Cellular Probes
0890-8508
Publisher
Elsevier
Volume
12
Issue
2
Identifiers
DOI: 10.1006/mcpr.1997.0152
Keywords
  • Activated Protein C Resistance
  • Factor Vleidenmutation
  • Amplification Refractory Mutation System
  • Thrombophilic Patients
  • Frequency Of The Mutation In The Normal Population
Disciplines
  • Biology
  • Design

Abstract

Abstract The authors have developed a simple and rapid amplification-refractory mutation system (ARMS) assay for the factor VLeidenmutation (MIM 227400.0001). Polymerase chain reaction (PCR) primers within exon 10 of the factor V gene were designed; one common upstream primer was paired with either a normal or a mutant-specific downstream primer. The assay was validated using factor VLeidenDNA samples of thrombophilic patients ascertained by the restriction enzyme cleavage method of reference. In the normal French population, allele frequencies of the factor VLeidenmutation are 2·6% for the Parisian region and 1·7% for the south of the country.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

ARMS test for diagnosis of factor V Leiden mutatio...

on Molecular and Cellular Probes April 1998

ARMS test for diagnosis of factor VLeiden mutation...

on Journal of Clinical Laboratory... 1996

[DNA polymorphism in a Brazilian population: relat...

on Revue française de transfusio... March 1992
More articles like this..