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Convenient method for the determination of picomole amounts of juvenile hormone

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
323
Issue
2
Identifiers
DOI: 10.1016/s0021-9673(01)90397-8

Abstract

Abstract From an aqueous methanol extract, both juvenile hormone (JH) and, as an internal standard, its ethyl ester are extracted into isooctane and adsorbed on alumina, washed free from accompanying material and then desorbed with dichloro-methane. After structural modification by addition of methanol, 10-hydroxy-11-methoxy-JH is adsorbed on alumina, again washed with dichloromethane to remove all the unchanged material and then desorbed. The next derivative obtained is 10-DMNFHS-11-methoxy-JH, which again is adsorbed on alumina, washed free from accompanying material, desorbed and then determined by combined gas chromatography-selected ion monitoring mass spectrometry. The versatility and high yield of this procedure are demonstrated for a series of insect species with JH contents from less than 1 up to 40 pmol/g fresh weight.

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