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Direct excision of 50 kb pair DNA fragments from megabase-sized fragments produced during apoptotic cleavage of genomic DNA

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
396
Identifiers
DOI: 10.1016/0014-5793(96)01130-1
Keywords
  • Apoptosis
  • Necrosis
  • High Molecular Weight Dna Fragmentation
  • Pulsed Field Gel Electrophoresis

Abstract

Abstract The DNA of U937 cells exposed to two different apoptotic stimuli, namely the cocktail H 2O 2/3-aminobenzamide (3AB) and etoposide, was analyzed using field inversion gel electrophoresis (FIGE) as well as programmable, autonomously controlled electrode electrophoresis (PACE). The results obtained indicate that FIGE is not appropriate for sizing apoptotic DNA fragments. PACE appears to be more accurate and reliable and the results obtained with this technique strongly suggest that the 50 kb DNA fragments are directly excised from Mb-sized DNA fragments without the intermediate cleavage of 200–300 kb products.

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