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Assessment of Equine Autoimmune Thrombocytopenia (EAT) by flow cytometry

BioMed Central
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  • Research Article
  • Medicine

Abstract BMC Blood Disorders (2001) 1:1 BMC Blood Disorders (2001) 1:1Research article Assessment of Equine Autoimmune Thrombocytopenia (EAT) by flow cytometry Rafael Nunez*1,4, M. Alice Gomes-Keller3, Colin Schwarzwald2 and Karsten Feige2 Address: 1Institutes of Virology, University of Zurich, Zurich, Switzerland, 2Department of Internal Medicine, University of Zurich, Zurich, Switzerland, 3Clinical Laboratory, University of Zurich, Zurich, Switzerland and 4Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, USA E-mail: Rafael Nunez* -; M. Alice Gomes-Keller -; Colin Schwarzwald -; Karsten Feige - *Corresponding author Abstract Rationale: Thrombocytopenia is a platelet associated process that occurs in human and animals as result of i) decreased production; ii) increased utilization; iii) increased destruction coupled to the presence of antibodies, within a process know as immune-mediated thrombocytopenia (IMT); or iv) platelet sequestration. Thus, the differentiation of the origin of IMT and the development of reliable diagnostic approaches and methodologies are important in the clarification of IMT pathogenesis. Therefore, there is a growing need in the field for easy to perform assays for assessing platelet morphological characteristics paired with detection of platelet-bound IgG. Objectives: This study is aimed to develop and characterize a single color flow cytometric assay for detection of platelet-bound IgG in horses, in combination with flow cytometric assessment of platelet morphological characteristics. Findings: The FSC and SSC evaluation of the platelets obtained from the thrombocytopenic animals shows several distinctive features in comparison to the flow cytometric profile of platelets from healthy animals. The thrombocytopenic animals displayed i) increased number of platelets with high

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