Affordable Access

Publisher Website

Structural Analysis of anEchinococcus granulosusActin-Fragmenting Protein by Small-Angle X-Ray Scattering Studies and Molecular Modeling

Authors
Journal
Biophysical Journal
0006-3495
Publisher
Elsevier
Publication Date
Volume
90
Issue
9
Identifiers
DOI: 10.1529/biophysj.105.067801
Keywords
  • Proteins
Disciplines
  • Biology

Abstract

Abstract The Echinococcus granulosus actin filament-fragmenting protein (EgAFFP) is a three domain member of the gelsolin family of proteins, which is antigenic to human hosts. These proteins, formed by three or six conserved domains, are involved in the dynamic rearrangements of the cytoskeleton, being responsible for severing and capping actin filaments and promoting nucleation of actin monomers. Various structures of six domain gelsolin-related proteins have been investigated, but little information on the structure of three domain members is available. In this work, the solution structure of the three domain EgAFFP has been investigated through small-angle x-ray scattering (SAXS) studies. EgAFFP exhibits an elongated molecular shape. The radius of gyration and the maximum dimension obtained by SAXS were, respectively, 2.52 ± 0.01 nm and 8.00 ± 1.00 nm, both in the absence and presence of Ca 2+. Two different molecular homology models were built for EgAFFP, but only one was validated through SAXS studies. The predicted structure for EgAFFP consists of three repeats of a central β-sheet sandwiched between one short and one long α-helix. Possible implications of the structure of EgAFFP upon actin binding are discussed.

There are no comments yet on this publication. Be the first to share your thoughts.