Abstract A novel Ni-free Ti47Cu38Zr7.5Fe2.5Sn2Si1Ag2 (at.%) bulk metallic glass (BMG) with superior glass-forming ability, good mechanical properties and excellent biocompatibility was discovered. The Ti-based BMG with a diameter of 7mm can be prepared by copper mold casting and the supercooled liquid region was 52K. Compressive strength, specific strength, Young’s modulus and microhardness of the Ti-based BMG were about 2.08GPa, 3.2×105Nm/kg, 100GPa and 588Hv, respectively. Electrochemical measurements indicated that the Ti-based glassy alloy possesses higher corrosion resistance than Ti–6Al–4V alloy in a simulated body fluid environment. Attachment, spreading out and proliferation of MC3T3-E1 cells on the Ti-based BMG surface demonstrated the excellent biocompatibility. Mechanisms of the formation and properties for the Ti-based glassy alloy are also discussed. The combination of high glass-forming ability, excellent mechanical properties, high corrosion resistance and good biocompatibility demonstrates the potential of the Ni-free Ti-based BMG for use in biomedical applications.