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Essential role of the first intron in the transcription of hsp90β gene

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
413
Issue
1
Identifiers
DOI: 10.1016/s0014-5793(97)00883-1
Keywords
  • Hsp90β Gene
  • Heat Shock Element (Hse)
  • The First Intron
  • Transcription Initiation Site
  • Chloramphenicol Acetyl Transferase (Cat)

Abstract

Abstract The human HSP90 gene family contains introns. There are two typical heat shock elements (HSE) in the first intron of human hsp90β gene. As detected by chloramphenicol acetyl transferase (CAT) reporter activity assays, the HSE-containing intron is essential in maintaining high constitutive expression and is critical for heat shock inducibility of the human hsp90β gene. Cellular heat shock factor 1 (HSF 1) shows much higher binding affinity toward the intronic HSEs in comparison to an atypical HSE in the 5′ flanking sequence. Novel initiation sites found in the first intron probably also contribute to constitutive and heat-inducible expression of the hsp90β gene in Jurkat cells.

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