Affordable Access

Publisher Website

An Assay for Simultaneous Multiple Determinations of Peptide Binding to MHC Class II Molecules

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
211
Issue
2
Identifiers
DOI: 10.1006/abio.1993.1268

Abstract

Abstract Binding of positively charged radiolabeled synthetic peptides to human major histocompatibility complex class II DR molecules, purified by affinity chromatography from lymphoblastoid B cell lines of different haplotypes, is rapidly, quantitatively, and specifically assayed by selective adsorption of the complexes between peptide and DR molecules onto DEAE-cellulose paper disks. This assay can be used as a revealing system of the ability of unlabeled test peptides to competitively inhibit the binding between the radiolabeled peptide and the DR molecules, thus measuring the binding of the competitor peptides, irrespective of their charge properties, to different DR molecules.

There are no comments yet on this publication. Be the first to share your thoughts.