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Nongenomic effects of estrogens on signal transduction pathways in estrogen-sensitive carcinoma cell lines

Breast Cancer Research
Springer (Biomed Central Ltd.)
Publication Date
DOI: 10.1186/bcr347
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abstracts.qxd Available online A1 The in vivo cell kinetics in breast carcinogenesis NJ Agnantis, SA Kamina, PS Zagorianakou, A Demou, A Katsaraki, P Kanavaros*, M Bai Department of Pathology, Medical School, University of Ioannina, Ioannina, Greece; *Department of Histology, Medical School, University of Thessalia, Larisa, Greece Background: Disruption of the balance between apoptosis and pro- liferation is considered to be an important factor in the development and progression of tumor. In this study we determined the in vivo cell kinetics along the spectrum of apparently normal epithelium, hyper- plasia, preinvasive lesions and invasive carcinoma, in breast tissues affected by fibrocystic changes in which preinvasive and/or invasive lesions developed, as a model of breast carcinogenesis. Materials and method: A total of 32 areas of apparently normal epithelium and 135 ductal proliferative and neoplastic lesions were studied. More than one epithelial lesion per case was analyzed. The apoptotic index (AI) and the proliferative index (PI) were expressed as the percentage of TUNEL (TdT-mediated dUTP-nick end-labelling) and Ki-67 positive cells, respectively. The prolifera- tive/apoptotic index (P/A) was calculated for each case. Results: Statistical analysis demonstrated significant differences among the tissue groups for both indices (P < 0.0001). The Als and PIs were significantly higher in hyperplasia than in apparently normal epithelium (P = 0.04 and P = 0.0005, respectively), in atypi- cal hyperplasia than in hyperplasia (P = 0.01 and P = 0.04, respec- tively) and in invasive carcinoma than in in situ carcinoma (P = 0.0001 and P < 0.0001, respectively). The two indices were similar in atypical hyperplasia and in in situ carcinoma. The P/A index increased significantly from normal epithelium to hyperplasia (P = 0.01) and from preinvasive lesions to invasive carcinoma (P = 0.04), whereas it was decreased (NS) from hyperplasia to preinvasive

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