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Effects of pH on erythrocyte metabolism

Authors
Journal
Archives of Biochemistry and Biophysics
0003-9861
Publisher
Elsevier
Publication Date
Volume
117
Issue
3
Identifiers
DOI: 10.1016/0003-9861(66)90087-7

Abstract

Abstract Anion-exchange analysis of nucleotides and other phosphate esters has been used to study the effects of pH on the levels of these organic phosphates during in vitro incubation of human erythrocytes for 5 hours. The pH of incubated samples was raised by ( a) permitting carbon dioxide to diffuse out of the sample and ( b), by addition of isotonic, pH 8.6 Tris buffer. In either case, the consequences of incubation at a pH of 7.6–7.7 were as follows: ( a) an increase in the AMP:ATP and ADP:ATP ratios; ( b) an increase in the level of hexose diphosphate; ( c) a decrease in the level of inorganic phosphate; and ( d) a marked increase in the rate of breakdown of adenine nucleotides to hypoxanthine. The probable causes for these changes are discussed.

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