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The importance of individual nucleotides for the structure and function of rRNA molecules inE. coli:A mutagenesis study

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
204
Issue
1
Identifiers
DOI: 10.1016/0014-5793(86)81392-8
Keywords
  • Ribosomal Rna
  • Mutagenesis
  • Protein-Rna Interaction
  • Trna Binding
  • Subunit Association
Disciplines
  • Biology

Abstract

Abstract Methods of in vitro mutagenesis were employed to determine the importance of individual nucleotides within the ribosomal RNAs for the structure and function of E. coli ribosomes. A series of denned nucleotides in the genes for the 5 S and 16 S RNA were altered by transition and transversion mutations using either oligonucleotide-directed or bisulfite-catalyzed mutation procedures. Plasmids harbouring the mutated rRNA genes were expressed and the ribosomes containing such altered RNAs were investigated for impairments in RNA-protein interaction assembly and mRNA-coded tRNA binding.

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