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Expression of protein IIIa of human adenovirus type 2 inEscherichia coli

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
55
Identifiers
DOI: 10.1016/0378-1119(87)90289-7
Keywords
  • Recombinant Dna
  • Capsid Protein
  • Tacpromoter
  • Protein Kinase
Disciplines
  • Biology

Abstract

Abstract We have constructed a plasmid encoding the protein IIIa gene of human adenovirus type 2. The gene was expressed under the control of the hybrid tac (trp-lac) promoter; the protein was synthesized at levels up to 5 % of newly synthesized protein after IPTG induction. The protein IIIa produced in Escherichia coli has an apparent M r on sodium dodecyl sulfate-polyacrylamide gels of 67 kDa, and was revealed with anti-adenovirus serum in Western blotting. The protein IIIa produced in bacteria was phosphorylated in the presence of [γ- 32P]ATP.

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