Affordable Access

Publisher Website

Cell-surface display of heterologous epitopes onStaphylococcus xylosusas a potential delivery system for oral vaccination

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
128
Issue
1
Identifiers
DOI: 10.1016/0378-1119(93)90158-y
Keywords
  • Recombinant Dna
  • Display Vectors
  • Gene Assembly
  • Class-Iis Restriction Enzyme
  • Bspmi
  • Recombinant Receptor
  • Staphylococcal Protein A
  • Gram-Positive Bacteria
  • Expression Vectors
  • Immunization
  • Serum Antibodies
Disciplines
  • Biology
  • Design

Abstract

Abstract A system has been developed for the surface expression of heterologous receptors on the cell surface of Staphylococcus xylosus. Gene fragments encoding peptides to be displayed on the cell surface can be assembled by applying a polymerization strategy based on the class-IIS restriction enzyme BspMI and thereafter subcloned into an Escherichia cohi-staphylococci shuttle vector designed for targeting of produced fusion proteins to the outer cell surface of the Gram-positive host cell. A heterologous receptor was genetically assembled and expressed on the surface of S. xylosus where the separate regions could be independently probed in immunogold assays, using rabbit antisera reacting with different regions of the recombinant receptor. In addition, a receptor-specific humoral immune response could be elicited in mice by oral immunization with recombinant S. xylosus cells, suggesting that these type of Gram-positive bacteria might offer potential vehicles for oral vaccination.

There are no comments yet on this publication. Be the first to share your thoughts.