Affordable Access

Publisher Website

Quantitative Proteome Profiling of C. burnetii under Tetracycline Stress Conditions

Authors
Journal
PLoS ONE
1932-6203
Publisher
Public Library of Science
Publication Date
Volume
7
Issue
3
Identifiers
DOI: 10.1371/journal.pone.0033599
Keywords
  • Research Article
  • Biology
  • Biochemistry
  • Microbiology
  • Bacterial Pathogens
  • Proteomics
  • Medicine
  • Drugs And Devices
  • Drug Research And Development
  • Infectious Diseases
  • Bacterial Diseases
Disciplines
  • Biology

Abstract

The recommended antibiotic regimen against Coxiella burnetii, the etiological agent of Q fever, is based on a semi-synthetic, second-generation tetracycline, doxycycline. Here, we report on the comparison of the proteomes of a C. burnetii reference strain either cultured under control conditions or under tetracycline stress conditions. Using the MS-driven combined fractional diagonal chromatography proteomics technique, out of the 531 proteins identified, 5 and 19 proteins were found significantly up- and down-regulated respectively, under tetracycline stress. Although the predicted cellular functions of these regulated proteins did not point to known tetracycline resistance mechanisms, our data clearly reveal the plasticity of the proteome of C. burnetii to battle tetracycline stress. Finally, we raise several plausible hypotheses that could further lead to more focused experiments on studying tetracycline resistance in C. burnetii and thus reduced treatment failures of Q fever.

There are no comments yet on this publication. Be the first to share your thoughts.