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Gain- and loss-of-function in chick embryos by electroporation

Authors
Journal
Mechanisms of Development
0925-4773
Publisher
Elsevier
Publication Date
Volume
121
Issue
9
Identifiers
DOI: 10.1016/j.mod.2004.05.013
Keywords
  • Chick Embryos
  • In Ovo
  • Electroporation
  • Sirna
  • Gain-Of-Function
  • Loss-Of-Function

Abstract

Abstract It remained very difficult to manipulate gene expression in chick embryos until the advent of in ovo electroporation which enabled the induction of both gain-of-function, and recently loss-of-function, of a gene of interest at a specific developmental stage. Gain-of-function by electroporation is so effective that it has become widely adopted in developmental studies in the chick. Recently, it became possible to induce loss-of-function by introducing an siRNA expression vector by electroporation. In this review, the methods of electroporation for gain-of-function and for loss-of-function by siRNA are discussed.

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