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β-Cyclodextrin and Oligoarginine Peptide-Based Dendrimer-Entrapped Gold Nanoparticles for Improving Drug Delivery to the Inner Ear

Authors
  • Luo, Jia1
  • Lin, XueXin1
  • Li, LiLing1
  • Tan, JingQian2
  • Li, Peng1
  • 1 The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou , (China)
  • 2 The Eighth Affiliated Hospital of Sun Yat-Sen University, Shenzhen , (China)
Type
Published Article
Journal
Frontiers in Bioengineering and Biotechnology
Publisher
Frontiers Media SA
Publication Date
Apr 11, 2022
Volume
10
Identifiers
DOI: 10.3389/fbioe.2022.844177
Source
Frontiers
Keywords
Disciplines
  • Bioengineering and Biotechnology
  • Original Research
License
Green

Abstract

Here, we developed a safe and highly effective nanocarrier using β-cyclodextrin (β-CD) and oligoarginine peptide (Arg8)-modified dendrimer-entrapped gold nanoparticles ([email protected]), with a diameter of 5 nm, for improved delivery of dexamethasone (Dex) to the inner ear. The properties and in vivo distribution of the [email protected] were assessed in vitro, and a streptomycin (SM) ototoxicity model was used in vivo. Flow cytometry analysis of HEIOC1 cells treated with [email protected] and Au @CD-PAMAM at different time intervals indicated that cell uptake efficiency of the drug delivery carrier [email protected] was higher than that of Au @CD-PAMAM. [email protected] carrying Dex ([email protected]/Dex) were mainly distributed in hair cells, the spiral ganglion, lateral wall, and nerve fibers and had stronger protective effects on the inner ear than Dex administration alone. In vivo tracer tests revealed that tympanic injection was significantly more effective than posterior ear injection, muscle injection, and tail vein injection, whereas clinical retro-auricular injection could not increase the efficiency of drug delivery into the ear. Electrocochleography results showed that [email protected]/Dex significantly improved hearing in C57/BL6 mice after SM exposure. These findings indicate that [email protected] may be the useful drug carriers for the treatment of inner ear diseases.

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