H. Li H. Zhang J.C. Hancox R.Z. Kozlowski
This report describes a hitherto unreported anionic background current from human atrial cardiomyocytes. Under whole-cell patch-clamp with anion-selective conditions, an outwardly rectifying anion current (IANION) was observed, which was larger with iodide than nitrate, and with nitrate than chloride as charge carrier. In contrast with a previously...
Satyajit D. Sarker Lutfun Nahar Yashodharan Kumarasamy
The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified ...
Mohammed Sebaihia Michael W. Peck Nigel P. Minton Nicholas R. Thomson Matthew T.G. Holden Wilfrid J. Mitchell Andrew T. Carter Stephen D. Bentley David R. Mason Lisa Crossman
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Clostridium botulinum is a heterogeneous Gram-positive species that comprises four genetically and physiologically distinct groups of bacteria that share the ability to produce botulinum neurotoxin, the most poisonous toxin known to man, and the causative agent of botulism, a severe disease of humans and animals. We report here the complete genome ...
Noah C. Welker Jeffrey W. Habig Brenda L. Bass
We describe the first microarray analysis of a whole animal containing a mutation in the Dicer gene. We used adult Caenorhabditis elegans and, to distinguish among different roles of Dicer, we also performed microarray analyses of animals with mutations in rde-4 and rde-1, which are involved in silencing by siRNA, but not miRNA. Surprisingly, we fi...
Jong Hyun Kim Michael S. Waterman Lei M. Li
One of the main goals in genome sequencing projects is to determine a haploid consensus sequence even when clone libraries are constructed from homologous chromosomes. However, it has been noticed that haplotypes can be inferred from genome assemblies by investigating phase conservation in sequenced reads. In this study, we seek to infer haplotypes...
Jong Hyun Kim Michael S. Waterman Lei M. Li
One of the main goals in genome sequencing projects is to determine a haploid consensus sequence even when clone libraries are constructed from homologous chromosomes. However, it has been noticed that haplotypes can be inferred from genome assemblies by investigating phase conservation in sequenced reads. In this study, we seek to infer haplotypes...
Katherine Belov Claire E. Sanderson Janine E. Deakin Emily S.W. Wong Daniel Assange Kaighin A. McColl Alex Gout Bernard de Bono Alexander D. Barrow Terence P. Speed
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The availability of the first marsupial genome sequence has allowed us to characterize the immunome of the gray short-tailed opossum (Monodelphis domestica). Here we report the identification of key immune genes, including the highly divergent chemokines, defensins, cathelicidins, and Natural Killer cell receptors. It appears that the increase in c...
Stephen J. Lolait Lesley Q. Stewart James A. Roper Gayle Harrison David S. Jessop W. Scott Young Anne-Marie O'Carroll
The arginine vasopressin (Avp) 1b receptor (Avpr1b) present on anterior pituitary corticotrophs is involved in the stimulation of adrenocorticotrophin (ACTH) secretion, especially during times of stress. Corticotrophin-releasing hormone (Crh) is considered the major ACTH secretagogue during acute stress while Avp appears to be the more dominant med...
Liran Carmel Igor B. Rogozin Yuri I. Wolf Eugene V. Koonin
Introns that interrupt eukaryotic protein-coding sequences are generally thought to be nonfunctional. However, for reasons still poorly understood, positions of many introns are highly conserved in evolution. Previous reconstructions of intron gain and loss events during eukaryotic evolution used a variety of simplified evolutionary models that yie...
Jong Hyun Kim Michael S. Waterman Lei M. Li
One of the main goals in genome sequencing projects is to determine a haploid consensus sequence even when clone libraries are constructed from homologous chromosomes. However, it has been noticed that haplotypes can be inferred from genome assemblies by investigating phase conservation in sequenced reads. In this study, we seek to infer haplotypes...