Miyazaki, Takatsugu Tanaka, Hidekazu Nakamura, Shuntaro Dohra, Hideo Funane, Kazumi
Published in
Journal of Applied Glycoscience
Dextran α-1,2-debranching enzyme (DDE) releases glucose with hydrolyzing α-(1→2)-glucosidic linkages in α-glucans, which are made up of dextran with α-(1→2)-branches and are generated by Leuconostoc bacteria. DDE was isolated from Microbacterium dextranolyticum (formerly known as Flavobacterium sp. M-73) 40 years ago, although the amino acid sequen...
Ito, Daichi Karita, Shuichi Umekawa, Midori
Published in
Journal of Applied Glycoscience
Lytic polysaccharide monooxygenases (LPMO) are key enzymes for the efficient degradation of lignocellulose biomass with cellulases. A lignocellulose-degradative strain, Paenibacillus xylaniclasticus TW1, has LPMO-encoding Px AA10A gene. Neither the C1/C4-oxidizing selectivity nor the enzyme activity of Px AA10A has ever been characterized. In this ...
Tsukida, Rikako Yoshida, Makoto Kaneko, Satoshi
Published in
Journal of Applied Glycoscience
Woody biomass is anticipated to be a resource for a decarbonized society, but the difficulty of isolating woody components is a significant challenge. Brown-rot fungi, a type of wood rotting fungi, decompose hemicellulose particularly efficiently. However, there are few reports on the hemicellulases from brown-rot fungi. An α-L-arabinofuranosidase ...
Kitaoka, Motomitsu
Published in
Journal of Applied Glycoscience
We created a Microsoft Excel file, Enzyme_Kinetics_Calculator, which includes macro programs that automatically calculates kinetic parameters for typical kinetic equations of enzymatic reactions, accompanied by their standard errors, by minimizing the residual sum of squares thereof. The [ S ]- v plot is automatically drawn with the theoretical lin...
Suzuki, Naoto Hanashiro, Isao Fujita, Naoko
Published in
Journal of Applied Glycoscience
Starch is comprised of very large α-glucan molecules composed primarily of linear amylose and highly branched amylopectin. Most methods for analyses of starch structure use hydrolytic enzymes to cleave starch. When undegraded, whole starch structures can be analyzed by gel-permeation chromatography (GPC), but this typically yields a single peak eac...
Awanthi, Mahanama Geegana Gamage Nagamoto, Saki Oku, Hirosuke Kitahara, Kanefumi Konishi, Teruko
Published in
Journal of Applied Glycoscience
Algal sulfated polysaccharides are known to be effective hyaluronidase inhibitors. We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera. Results showed that SP with IC50 of 163 µg/mL appears to allosterically inhibit the hyaluronidase activity. Main sugar composition and sulfate content of SP was...
Tokuyasu, Ken Yamagishi, Kenji Ando, Yasumasa Shirai, Nobuya
Published in
Journal of applied glycoscience
Cabbage core (CC) is regarded as a waste part of the vegetable, despite being edible and containing various nutritional and functional compounds. We investigated the properties of CC powder with particle sizes
Tsutsui, Sosyu Hatano, Tomohiro Funada, Ryo Kaneko, Satoshi
Published in
Journal of applied glycoscience
In recent years, the importance of biomass utilization has increased, but it has not been effectively exploited. In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass. Therefore, in order to promote the utilization of hemicellulose, we screened for microorganisms capable of producing polysaccharides fro...
Shintani, Tomoya Yanai, Shuichi Kanasaki, Akane Tanaka, Misuzu Iida, Tetsuo Ozawa, Genki Kunihiro, Tadao Endo, Shogo
Published in
Journal of applied glycoscience
D-Allose, a C3 epimer of D-glucose, has potential to improve human health as a functional food. However, its effect on the intestinal environment remains unknown. Aged humans progressively express changes in the gut, some of which deleteriously affect gastrointestinal health. In this study, we profiled the intestinal microbiome in aged mice and ana...
Tokuyasu, Ken Matsuki, Junko Yamagishi, Kenji Ike, Masakazu
Published in
Journal of applied glycoscience
This study aimed to characterize the interactions between cereal flour (rice, wheat, and barley) and "nata puree" (NP), a disintegrated bacterial cellulose (BC) in the presence of a water-soluble polysaccharide, with powder-dispersion activity. Pasting properties of cereal flour with additives were analyzed using a Rapid Visco Analyzer, and disinte...
