Guilbert, Jérémie Légaré, Antoine De Koninck, Paul Desrosiers, Patrick Desjardins, Michèle
Published in
Neurophotonics
Brain functional connectivity based on the measure of blood oxygen level-dependent (BOLD) functional magnetic resonance imaging (fMRI) signals has become one of the most widely used measurements in human neuroimaging. However, the nature of the functional networks revealed by BOLD fMRI can be ambiguous, as highlighted by a recent series of experime...
Chao, Tzu-Hao H Zhang, Wei-Ting Hsu, Li-Ming Cerri, Domenic H Wang, Tzu-Wen Shih, Yen-Yu I
Published in
Neurophotonics
Significance: Although emerging evidence suggests that the hemodynamic response function (HRF) can vary by brain region and species, a single, canonical, human-based HRF is widely used in animal studies. Therefore, the development of flexible, accessible, brain-region specific HRF calculation approaches is paramount as hemodynamic animal studies be...
Bojovic, Danica Stackhouse, Teresa L Mishra, Anusha
Published in
Neurophotonics
Significance: Neurovascular coupling (NVC) is the process that increases cerebral blood flow in response to neuronal activity. NVC is orchestrated by signaling between neurons, glia, and vascular cells. Elucidating the mechanisms underlying NVC at different vascular segments and in different brain regions is imperative for understanding of brain fu...
Meyer-Baese, Lisa Watters, Harrison Keilholz, Shella
Published in
Neurophotonics
The brain exists in a state of constant activity in the absence of any external sensory input. The spatiotemporal patterns of this spontaneous brain activity have been studied using various recording and imaging techniques. This has enabled considerable progress to be made in elucidating the cellular and network mechanisms that are involved in the ...
Barkaway, Anna Attwell, David Korte, Nils
Published in
Neurophotonics
Brain barriers are crucial sites for cerebral energy supply, waste removal, immune cell migration, and solute exchange, all of which maintain an appropriate environment for neuronal activity. At the capillary level, where the largest area of brain-vascular interface occurs, pericytes adjust cerebral blood flow (CBF) by regulating capillary diameter...
Coelho-Santos, Vanessa Tieu, Taryn Shih, Andy Y
Published in
Neurophotonics
Significance: Two-photon microscopy is a powerful tool for in vivo imaging of the mammalian brain at cellular to subcellular resolution. However, resources that describe methods for imaging live newborn mice have remained sparse. Aim: We describe a non-invasive cranial window procedure for longitudinal imaging of neonatal mice. Approach: We demonst...
Freitas-Andrade, Moises Comin, Cesar H da Silva, Matheus Viana Costa, Luciano da F Lacoste, Baptiste
Published in
Neurophotonics
Significance: A growing body of research supports the significant role of cerebrovascular abnormalities in neurological disorders. As these insights develop, standardized tools for unbiased and high-throughput quantification of cerebrovascular structure are needed. Aim: We provide a detailed protocol for performing immunofluorescent labeling of mou...
Faulhaber, Lila D D'Costa, Olivia Shih, Andy Y Gust, Juliane
Published in
Neurophotonics
Significance: To study leukocyte-endothelial interactions in a living system, robust and specific leukocyte labeling techniques are needed for in vivo two-photon microscopy of the cerebral microvasculature. Aim: We tested fluorophore-conjugated anti-CD45.2 monoclonal antibodies (mAb) to optimize dosing and two-photon imaging parameters for leukocyt...
Yin, Rongkang Noble, Brian C He, Fei Zolotavin, Pavlo Rathore, Haad Jin, Yifu Sevilla, Nicole Xie, Chong Luan, Lan
Published in
Neurophotonics
Significance: Electrophysiological recording and optical imaging are two prevalent neurotechnologies with complementary strengths, the combined application of which can significantly improve our capacity in deciphering neural circuits. Flexible electrode arrays can support longitudinal optical imaging in the same brain region, but their mechanical ...
Lambers, Henriette Wachsmuth, Lydia Thomas, Dominik Boumezbeur, Fawzi Hoesker, Vanessa Pradier, Bruno Faber, Cornelius
Published in
Neurophotonics
Significance: Fluorescence resonance energy transfer (FRET) sensors offer enormous benefits when studying neurophysiology through confocal microscopy. Yet, their use for fiber-based in vivo recordings is hampered by massive confounding effects and has therefore been scarcely reported. Aim: We aim to investigate whether in vivo fiber-based lactate r...