Shaygan, Maryam Yazdanpanah, Maryam
Published in
The international journal of occupational and environmental medicine
Taking into account the differences in job requirements and conditions, it is expected that workers in some occupations are more susceptible to pain than others. To examine the prevalence of chronic pain among workers of several petrochemical and petroleum refinery plants. We also examined the predictive role of psycho-familial variables (depressio...
Marion STRULLU Caye, Aurélie Lachenaud, Julie Bruno Cassinat Gazal, Steven Fenneteau, Odile Pouvreau, Nathalie Pereira, Sabrina Baumann, Clarisse Contet, Audrey
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Published in
Journal of Visualized Experiments
JMML represents the first cause of death in PTPN11-associated NS. Few patients have been reported so far, suggesting that JMML may sometimes be overlooked due to early death, comorbidities or lack of confirmatory tests.
Maeß, Marten B Wittig, Berith Stefan Lorkowski
Published in
Journal of Visualized Experiments
Macrophages, as key players of the innate immune response, are at the focus of research dealing with tissue homeostasis or various pathologies. Transfection with siRNA and plasmid DNA is an efficient tool for studying their function, but transfection of macrophages is not a trivial matter. Although many different approaches for transfection of euka...
Muller, Mandy Cassonnet, Patricia Favre, Michel Yves Jacob Demeret, Caroline
Published in
Journal of Visualized Experiments
Significant efforts were gathered to generate large-scale comprehensive protein-protein interaction network maps. This is instrumental to understand the pathogen-host relationships and was essentially performed by genetic screenings in yeast two-hybrid systems. The recent improvement of protein-protein interaction detection by a Gaussia luciferase-...
Gonzalez-Hernandez, Mariam B Bragazzi Cunha, Juliana Wobus, Christiane E
Published in
Journal of visualized experiments : JoVE
Murine norovirus (MNV) is the only member of the Norovirus genus that efficiently grows in tissue culture. Cell lysis and cytopathic effect (CPE) are observed during MNV-1 infection of murine dendritic cells or macrophages. This property of MNV-1 can be used to quantify the number of infectious particles in a given sample by performing a plaque ass...
Wong, Chung Vosburgh, Evan Levine, Arnold J Cong, Lei Xu, Eugenia Y
Published in
Journal of visualized experiments : JoVE
Neuroendocrine tumors (NETs) are rare tumors, with an incidence of two per 100, 000 individuals per year, and they account for 0.5% of all human malignancies. Other than surgery for the minority of patients who present with localized disease, there is little or no survival benefit of systemic therapy. Therefore, there is a great need to better unde...
Salmon, Hélène Rivas-Caicedo, Ana Asperti-Boursin, François Lebugle, Camille Pierre Bourdoncle Emmanuel Donnadieu
Published in
Journal of Visualized Experiments
This protocol describes a method to image fluorescent T cells introduced into lymph node slices. The technique permits real-time analyses of T cell migration with traditional widefield fluorescence or confocal microscopes.
Ali, Yousuf O Escala, Wilfredo Ruan, Kai Zhai, R Grace
Published in
Journal of visualized experiments : JoVE
Advances in genetic methods have enabled the study of genes involved in human neurodegenerative diseases using Drosophila as a model system. Most of these diseases, including Alzheimer's, Parkinson's and Huntington's disease are characterized by age-dependent deterioration in learning and memory functions and movement coordination. Here we use beha...
Zlatic, Stephanie A Ryder, Pearl V Salazar, Gloria Faundez, Victor
Published in
Journal of visualized experiments : JoVE
The dynamic nature of cellular machineries is frequently built on transient and/or weak protein associations. These low affinity interactions preclude stringent methods for the isolation and identification of protein networks around a protein of interest. The use of chemical crosslinkers allows the selective stabilization of labile interactions, th...
Y Jeune - Smith Ashutosh Agarwal H Hess
Published in
Journal of Visualized Experiments
