Characterization of MDAPhi, a temperate filamentous bacteriophage of Neisseria meningitidis
Published in Microbiology
Detection of potential microbial antigens by immuno-PCR (PCR-amplified immunoassay).
Published in Journal of Medical Microbiology
Immuno-PCR (PCR-amplified immunoassay; I-PCR) is a novel ultrasensitive method combining the versatility of ELISA with the sensitivity of nucleic acid amplification of PCR. The enormous exponential amplification power of PCR in an I-PCR assay leads to at least a 10(2)-10(4)-fold increase in sensitivity compared with an analogous ELISA. I-PCR has be...
The prokaryotic community of subglacial bottom sediments of Antarctic Lake Untersee: Detection by cultural and direct mi...
Published in Microbiology
The heterotrophic mesophilic microbial component was studied in microbial communities of the samples of frozen regolith collected from the glacier near Lake Untersee collected in 2011 during the joint Russian-American expedition to central Dronning Maud Land (Eastern Antarctica). Cultural techniques revealed high bacterial numbers in the samples. F...
Identification of the domains of cauliflower mosaic virus protein P6 responsible for suppression of RNA silencing and sa...
...Published in The Journal of general virology
Cauliflower mosaic virus (CaMV) encodes a 520 aa polypeptide, P6, which participates in several essential activities in the virus life cycle including suppressing RNA silencing and salicylic acid-responsive defence signalling. We infected Arabidopsis with CaMV mutants containing short in-frame deletions within the P6 ORF. A deletion in the distal e...
Detection of phage infection in the bacterial population of Lake Untersee (Antarctica)
Published in Microbiology
FILIPPOVA et al. absence of oxygen at a depth of 80–90 m, the concen tration of dissolved methane reaches 21 mM [10]. The high pH of this mixed water mass (pH 10.2–6.9) has been attributed to weathering of the predominant anorthosite rock in the lake s hollow [11, 12]. The benthic environment is dominated by cyanobacterial mats forming cuspate pinn...
Pythium oligandrum: an example of opportunistic success.
Published in Microbiology
Pythium oligandrum, a non-pathogenic soil-inhabiting oomycete, colonizes the root ecosystem of many crop species. Whereas most members in the genus Pythium are plant pathogens, P. oligandrum distinguishes itself from the pathogenic species by its ability to protect plants from biotic stresses in addition to promoting plant growth. The success of P....
The cell envelope stress response mediated by the LiaFSRLm three-component system of Listeria monocytogenes is controlle...
Published in Microbiology
Most members of the phylum Firmicutes harbour a two-component system (TCS), LiaSR, which is involved in the response to cell envelope stress elicited most notably by inhibitors of the lipid II cycle. In all LiaSR systems studied in detail, LiaSR-mediated signal transduction has been shown to be negatively controlled by a membrane protein, LiaF, enc...
Inner tegument protein pUL37 of herpes simplex virus type 1 is involved in directing capsids to the trans-Golgi network ...
Published in The Journal of general virology
Secondary envelopment of herpes simplex virus type 1 has been demonstrated as taking place at the trans-Golgi network (TGN). The inner tegument proteins pUL36 and pUL37 and the envelope glycoproteins gD and gE are known to be important for secondary envelopment. We compared the cellular localizations of capsids from a virus mutant lacking the UL37 ...
Aerosol and nasal transmission of chronic wasting disease in cervidized mice.
Published in The Journal of general virology
Little is known regarding the potential risk posed by aerosolized prions. Chronic wasting disease (CWD) is transmitted horizontally, almost surely by mucosal exposure, and CWD prions are present in saliva and urine of infected animals. However, whether CWD may be transmissible by the aerosol or nasal route is not known. To address this question, FV...
Characterization of a novel wood mouse virus related to murid herpesvirus 4.
...Published in The Journal of general virology
Two novel gammaherpesviruses were isolated, one from a field vole (Microtus agrestis) and the other from wood mice (Apodemus sylvaticus). The genome of the latter, designated wood mouse herpesvirus (WMHV), was completely sequenced. WMHV had the same genome structure and predicted gene content as murid herpesvirus 4 (MuHV4; murine gammaherpesvirus 6...
