Jemth, Ann-Sofie Scaletti, Emma Rose Homan, Evert Stenmark, Pål Helleday, Thomas Michel, Maurice
Published in
The Journal of biological chemistry
Remdesivir and molnupiravir have gained considerable interest because of their demonstrated activity against SARS-CoV-2. These antivirals are converted intracellularly to their active triphosphate forms remdesivir-TP and molnupiravir-TP. Cellular hydrolysis of these active metabolites would consequently decrease the efficiency of these drugs; howev...
Liu, Zi Huang, Liancheng Zhou, Tianhao Chang, Xing Yang, Yuying Shi, Yani Hao, Mingjing Li, Zengqiang Wu, Yingliang Guan, Qi
...
Published in
The Journal of biological chemistry
Tumor angiogenesis is closely associated with the metastasis and progression of non-small cell lung cancer (NSCLC), a highly vascularized solid tumor. However, novel therapeutics are lacking for the treatment of this cancer. Here, we developed a series of 2-aryl-4-(3,4,5-trimethoxy-benzoyl)-5-substituted-1,2,3-triazol analogs (6a-6x) as tubulin col...
Zhang, Xiaohan Malik, Bhoomanyu Young, Crystal Zhang, Hao Larkin, Dennis Liao, Xiao-Hui Refetoff, Samuel Liu, Ming Arvan, Peter
Published in
The Journal of biological chemistry
Congenital hypothyroidism with biallelic thyroglobulin (Tg protein, encoded by the TG gene) mutation is an endoplasmic reticulum (ER) storage disease. Many patients (and animal models) grow an enlarged thyroid (goiter), yet some do not. In adulthood, hypothyroid TGcog/cog mice (bearing a Tg-L2263P mutation) exhibit a large goiter, whereas adult WIC...
Hao, Binhan Zhou, Wenjie Theg, Steven M
Published in
The Journal of biological chemistry
The twin-arginine translocation (Tat) pathway transports folded proteins across membranes in bacteria, thylakoids, plant mitochondria, and archaea. In most species, the active Tat machinery consists of three independent subunits: TatA, TatB, and TatC. TatA and TatB possess short transmembrane alpha helices (TMHs), both of which are only 15 residues...
Akimoto, Miho Susa, Takao Okudaira, Noriyuki Hisaki, Harumi Iizuka, Masayoshi Okinaga, Hiroko Okazaki, Tomoki Tamamori-Adachi, Mimi
Published in
The Journal of biological chemistry
Long noncoding RNAs (lncRNAs) are important tissue-specific regulators of gene expression, and their dysregulation can induce aberrant gene expression leading to various pathological conditions, including cancer. Although many lncRNAs have been discovered by computational analysis, most of these are as yet unannotated. Herein, we describe the natur...
FitzGerald, Garret A.
Published in
Journal of Lipid Research
Gonzalez-Gil, Anabel Porell, Ryan N Fernandes, Steve M Maenpaa, Eila Li, T August Li, Tong Wong, Philip C Aoki, Kazuhiro Tiemeyer, Michael Yu, Zaikuan J
...
Published in
The Journal of biological chemistry
Alzheimer's disease (AD) is characterized by accumulation of misfolded proteins. Genetic studies implicate microglia, brain-resident phagocytic immune cells, in AD pathogenesis. As positive effectors, microglia clear toxic proteins, whereas as negative effectors, they release proinflammatory mediators. An imbalance of these functions contributes to...
Hernandez, Alfredo J Lee, Seung-Joo Thompson, Noah J Griffith, Jack D Richardson, Charles C
Published in
The Journal of biological chemistry
The T7 primase-helicase plays a pivotal role in the replication of T7 DNA. Using affinity isolation of peptide-nucleic acid crosslinks and mass spectrometry, we identify protein regions in the primase-helicase and T7 DNA polymerase that form contacts with the RNA primer and DNA template. The contacts between nucleic acids and the primase domain of ...
Liu, Junhao Zhao, Yang Ding, Zhao Zhao, Yue Chen, Tingting Ge, Wenhao Zhang, Jianfa
Published in
The Journal of biological chemistry
Iron accumulates with age in mammals, and its possible implications in altering metabolic responses are not fully understood. Here, we report that both high-iron diet and advanced age in mice consistently altered gene expression of many pathways, including those governing the oxidative stress response and the circadian clock. We used a metabolomic ...
Li, Yang Huang, Shijia Wang, Jingsi Dai, Jianli Cai, Jie Yan, Shuai Huang, Zhiliang He, Shengqi Wang, Ping Liu, Jianmiao
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Published in
The Journal of biological chemistry
Inositol-requiring enzyme 1 (IRE1) is an evolutionarily conserved sensor of endoplasmic reticulum (ER) stress and mediates a key branch of the unfolded protein response in eukaryotic cells. It is an ER-resident transmembrane protein that possesses Ser/Thr protein kinase and endoribonuclease (RNase) activities in its cytoplasmic region. IRE1 is acti...
