Puthenveetil, Robbins Lun, Cheng Man Murphy, R Elliot Healy, Liam B Vilmen, Geraldine Christenson, Eric T Freed, Eric O Banerjee, Anirban
Published in
The Journal of biological chemistry
S-acylation, also known as palmitoylation, is the most widely prevalent form of protein lipidation, whereby long-chain fatty acids get attached to cysteine residues facing the cytosol. In humans, 23 members of the zDHHC family of integral membrane enzymes catalyze this modification. S-acylation is critical for the life cycle of many enveloped virus...
Jacoby, Christian Goerke, Malina Bezold, Dominik Jessen, Henning Boll, Matthias
Published in
The Journal of biological chemistry
The degradation of cholesterol and related steroids by microbes follows fundamentally different strategies in aerobic and anaerobic environments. In anaerobic bacteria, the primary C26 of the isoprenoid side chain is hydroxylated without oxygen via a three-step cascade: (i) water-dependent hydroxylation at the tertiary C25, (ii) ATP-dependent dehyd...
Nguyen, Phong Quoc Conesa, Christine Rabut, Elise Bragagnolo, Gabriel Gouzerh, Célia Fernández-Tornero, Carlos Lesage, Pascale Reguera, Juan Acker, Joël
Published in
The Journal of Biological Chemistry
Long-terminal repeat (LTR) retrotransposons are genetic elements that, like retroviruses, replicate by reverse transcription of an RNA intermediate into a complementary DNA (cDNA) that is next integrated into the host genome by their own integrase. The Ty1 LTR retrotransposon has proven to be a reliable working model to investigate retroelement int...
Zhao, Yang Cheng, Rui Zhao, Yue Ge, Wenhao Yang, Yunxia Ding, Zhao Xu, Xi Wang, Zhongqiu Wu, Zhenguo Zhang, Jianfa
...
Published in
The Journal of biological chemistry
Hibernation is an example of extreme hypometabolic behavior. How mammals achieve such a state of suspended animation remains unclear. Here we show that several strains of type 2 diabetic mice spontaneously enter into hibernation-like suspended animation (HLSA) in cold temperatures. Nondiabetic mice injected with ATP mimic the severe hypothermia ana...
Salsaa, Michael Aziz, Kerestin Lazcano, Pablo Schmidtke, Michael W Tarsio, Maureen Hüttemann, Maik Reynolds, Christian A Kane, Patricia M Greenberg, Miriam L
Published in
The Journal of biological chemistry
Valproate (VPA) is a widely used mood stabilizer, but its therapeutic mechanism of action is not understood. This knowledge gap hinders the development of more effective drugs with fewer side effects. Using the yeast model to elucidate the effects of VPA on cellular metabolism, we determined that the drug upregulated expression of genes normally re...
Zhang, Fengwu Zhao, Yongchao Sun, Yi
Published in
The Journal of biological chemistry
The stability of a protein is regulated by a balance between its ubiquitylation and deubiquitylation. S-phase kinase-associated protein 2 (SKP2) is an oncogenic F-box protein that recognizes tumor suppressor substrates for targeted ubiquitylation by the E3 ligase SKP1-Cullin1-F-box and degradation by proteasome. SKP2 is itself ubiquitylated by the ...
Pong, Carol H Harmer, Christopher J Flores, Janine K Ataide, Sandro F Hall, Ruth M
Published in
The Journal of biological chemistry
The bacterial insertion sequence (IS) IS26 mobilizes and disseminates antibiotic resistance genes. It differs from bacterial IS that have been studied to date as it exclusively forms cointegrates via either a copy-in (replicative) or a recently discovered targeted conservative mode. To investigate how the Tnp26 transposase recognizes the 14-bp term...
Fu, Nae Sugiura, Kazunori Kondo, Kumiko Nakamura, Shungo Wakabayashi, Ken-Ichi Hisabori, Toru
Published in
The Journal of biological chemistry
Reactive oxygen species are key factors that strongly affect the cellular redox state and regulate various physiological and cellular phenomena. To monitor changes in the redox state, we previously developed fluorescent redox sensors named Re-Q, the emissions of which are quenched under reduced conditions. However, such fluorescent probes are unsui...
Guo, Yusong Liu, Qi Mallette, Evan Caba, Cody Hou, Feng Fux, Julia LaPlante, Gabriel Dong, Aiping Zhang, Qi Zheng, Hui
...
Published in
The Journal of biological chemistry
Ubiquitination is a crucial posttranslational protein modification involved in a myriad of biological pathways. This modification is reversed by deubiquitinases (DUBs) that deconjugate the single ubiquitin (Ub) moiety or poly-Ub chains from substrates. In the past decade, tremendous efforts have been focused on targeting DUBs for drug discovery. Ho...
Dai, Dan-Ling Li, Xingyang Wang, Lin Xie, Chu Jin, Yanan Zeng, Mu-Sheng Zuo, Zhixiang Xia, Tian-Liang
Published in
The Journal of Biological Chemistry
