Freire-Paspuel, Byron Garcia-Bereguiain, Miguel Angel
Published in
International Journal of Infectious Diseases
Background Several RT-qPCR kits are available for SARS-CoV-2 diagnosis, some of them with Emergency Use Authorization (EUA) by FDA like de nCoV19 CDC kit that includes 2 targets for detection of SARS-CoV-2 (N1, N2) and one target for RNA extraction quality control (RP), all them labelled with FAM so 3 PCR reactions are required per sample. Methods ...
Calvo-Lozano, Olalla Aviñó, Anna Friaza, Vicente Medina-Escuela, Alfonso S. Huertas, César Calderón, Enrique J. Eritja, Ramón Lechuga, Laura M.
Published in
Nanomaterials
Pneumocystis jirovecii is a fungus responsible for human Pneumocystis pneumonia, one of the most severe infections encountered in immunodepressed individuals. The diagnosis of Pneumocystis pneumonia continues to be challenging due to the absence of specific symptoms in infected patients. Moreover, the standard diagnostic method employed for its dia...
Herbert, Alan
Published in
Royal Society open science
ALUs contribute to genetic diversity by altering DNA's linear sequence through retrotransposition, recombination and repair. ALUs also have the potential to form alternative non-B-DNA conformations such as Z-DNA, triplexes and quadruplexes that alter the read-out of information from the genome. I suggest here these structures enable the rapid repro...
O’Neill, Rachel J.
Published in
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Innovations in high throughout sequencing approaches are being marshaled to both reveal the composition of the abundant and heterogeneous noncoding RNAs that populate cell nuclei and lend insight to the mechanisms by which noncoding RNAs influence chromosome biology and gene expression. This review focuses on some of the recent technological develo...
Kitamura, Yusuke Nagai, Koki Furuzono, Tomohiro Katsuda, Yousuke Ihara, Toshihiro
Published in
Nucleosides, nucleotides & nucleic acids
Cooperative recognition of a repetitive sequence was performed with a short single DNA strand consisting of duplex- and triplex-forming regions modified with a ligand (benzoquinoquinoxaline) to stabilize a triplex structure. The former region was complementary with one unit of a repetitive sequence and the latter had a sequence that can bind with a...
Fu, Wenhao Tang, Linlin Wei, Gaohui Fang, Liang Zeng, Jie Zhan, Renjie Liu, Xuemei Zuo, Hua Huang, Cheng Zhi Mao, Chengde
...
Published in
Angewandte Chemie (International ed. in English)
Reconfigurable molecular events are key to molecular machines. In response to external cues, molecular machines rearrange/change their structures to perform certain functions. Such machines exist in nature, for example cell surface receptors, and have been artificially engineered. To be able to build sophisticated and efficient molecular machines f...
Naskar, Shuvankar Guha, Rweetuparna Müller, Jens
Published in
Angewandte Chemie (International ed. in English)
The incorporation of metal ions into nucleic acids by means of metal-mediated base pairs represents a promising and prominent strategy for the site-specific decoration of these self-assembling supramolecules with metal-based functionality. Over the past 20 years, numerous nucleoside surrogates have been introduced in this respect, broadening the me...
Aviñó, Anna Eritja, Ramon Ciudad, Carlos J Noé, Verónica
Published in
Current protocols in nucleic acid chemistry
Nucleic acid triplexes are formed when a DNA or RNA oligonucleotide binds to a polypurine-polypyrimidine-rich sequence. Triplexes have wide therapeutic applications such as gene silencing or site-specific mutagenesis. In addition, protocols based on triplex-affinity capture have been used for detecting nucleic acids in biosensing platforms. In this...
Kuo, Chao-Chung
Gene expression complexity is correlated to the proportion of the non-coding part of the genome. This fact implies the potential ability of non-coding elements to regulate gene expression in addition to the paradigm of protein-based regulation model. Among these non-coding elements, long non-coding RNAs (lncRNAs) can act as scaffolds that promote t...
Wang, Fan Chainani, Pranik White, Tommy Yang, Jin Liu, Yu Soibam, Benjamin
Published in
RNA biology
Long noncoding RNAs (lncRNAs) can exert their function by interacting with the DNA via triplex structure formation. Even though this has been validated with a handful of experiments, a genome-wide analysis of lncRNA-DNA binding is needed. In this paper, we develop and interpret deep learning models that predict the genome-wide binding sites deciphe...
