A (p.?). Blood TPP levels were reduced, providing functional evidence for the deleterious effects of the variants. We highlight the clinical and bioinformatics challenges to diagnosing rare genetic disorders and the continued utility, despite major advances in DNA sequencing technology, of biochemical analyses when investigating novel, potentially ...
Published in American journal of physiology. Cell physiology
Microbiota of the large intestine synthesize considerable amount of vitamin B1 in the form of thiamine pyrophosphate (TPP). There is a specific high-affinity regulated carrier-mediated uptake system for TPP in human colonocytes (product of the SLC44A4 gene). The mechanisms of regulation of SLC44A4 gene expression are currently unknown. In this stud...
Published in Journal of the Chinese Medical Association : JCMA
This study investigated the effect of thiamine pyrophosphate (TPP) on oxidative liver damage induced in rats with high-dose paracetamol. Rats for this experiment were divided into the following groups: healthy control, paracetamol control, thiamine + paracetamol, TPP + paracetamol, and N-acetylcysteine + paracetamol. Oxidant and antioxidant paramet...
Published in Pharmaceutical Research
PurposeChitosan, a natural and biocompatible cationic polymer, is an attractive carrier for small interfering RNA (siRNA) delivery. The purpose of this study was to develop a chitosan-based hybrid nanocomplex that exhibits enhanced physical stability in the bloodstream compared with conventional chitosan complexes. Hybrid nanocomplexes composed of ...
Published in International Journal of Hydrogen Energy
•We express an active [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii.•The chloroplast hydrogenase is no longer regulated by the native control system.•Production of the apoprotein occurs under both aerobic and anaerobic conditions.•Unregulated expression results in a strong negative selective pressure.•A vitamin-controlled gene ...
Published in Phytochemistry
Glyoxylate is a peroxisomal intermediate of photorespiration, the recycling pathway for 2-phosphoglycolate (2-PG) produced by the oxygenase activity of Rubisco. Under hot and dry growth conditions, photorespiratory intermediates can accumulate and must be detoxified by alternative pathways, including plastidal reactions. Moreover, there is evidence...
Published in Indian journal of pharmacology
The biochemical effects of thiamine pyrophosphate on ischemia-reperfusion (IR) induced oxidative damage and DNA mutation in rat kidney tissue were investigated, and compared to thiamine. Rats were divided into four groups: renal ischemia-reperfusion (RIR); thiamine pyrophosphate + RIR (TPRIR); thiamine + RIR (TRIR); and sham group (SG). The results...
Published in Russian Journal of Plant Physiology
The THIC gene involved in thiamin biosynthesis in arabidopsis (Arabidopsis thaliana (L.) Heynh) encodes two mRNAs produced by alternative splicing; the ratio between these mRNAs is controlled by the changing thiamine pyrophosphate (TPP) concentration in the plant. One of produced splicing isoforms (THIC-III) is subjected to rapid degradation, which...
Published in Comprehensive Natural Products II: Chemistry and Biology
Posttranslational modifications diversify the limited amino acid pool, expanding 20 amino acids into a possibly limitless number of residues. These modifications are catalyzed by a large family of proteins specific to each modification. This chapter focuses on the function of common posttranslational modifications and highlights work done in the la...
Published in Encyclopedia of Microbiology
Central metabolic pathways are those biochemical pathways used by organisms to produce utilizable energy and the biosynthetic precursors and intermediates needed by the cell. In microorganisms, these pathways can include the Embden–Meyerhof–Parnas pathway, the pentose phosphate pathway (PPP), the Entner–Doudoroff pathway, and the pentose phosphoket...
