Liu, Yuqi Chen, Yundai
Published in
Frontiers in Cell and Developmental Biology
Essential hypertension (EH) is one of the most common cardiovascular diseases worldwide, entailing a high level of morbidity. EH is a multifactorial disease influenced by both genetic and environmental factors, including mitochondrial DNA (mtDNA) genotype. Previous studies identified mtDNA mutations that are associated with maternally inherited hyp...
Joshi, Bishnu Singh, Bhupender Nadeem, Aftab Askarian, Fatemeh Wai, Sun Nyunt Johannessen, Mona Hegstad, Kristin
Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureus MSSA476 cultured in iron-depleted bacteriologic ...
Croitoru, Anastasia Babin, Morgan Myllykallio, Hannu Gondry, Muriel Aleksandrov, Alexey
Published in
Biochemistry
Cyclodipeptide synthases (CDPSs) perform nonribosomal protein synthesis using two aminoacyl-tRNA substrates to produce cyclodipeptides. At present, there are no structural details of the CDPS:tRNA interaction available. Using AlbC, a CDPS that produces cyclo(l-Phe-l-Phe), the interaction between AlbC and its Phe-tRNA substrate was investigated. Sim...
Samatova, Ekaterina Daberger, Jan Liutkute, Marija Rodnina, Marina V.
Published in
Frontiers in Microbiology
Protein homeostasis of bacterial cells is maintained by coordinated processes of protein production, folding, and degradation. Translational efficiency of a given mRNA depends on how often the ribosomes initiate synthesis of a new polypeptide and how quickly they read the coding sequence to produce a full-length protein. The pace of ribosomes along...
PICHOT, Florian MARCHAND, Virginie HELM, Mark MOTORIN, Yuri
Published in
Genes
Analysis of RNA by deep-sequencing approaches has found widespread application in modern biology. In addition to measurements of RNA abundance under various physiological conditions, such techniques are now widely used for mapping and quantification of RNA modifications. Transfer RNA (tRNA) molecules are among the frequent targets of such investiga...
Arroyo, Maria Nicol Green, Jonathan Alex Cnop, Miriam Igoillo-Esteve, Mariana
Published in
International journal of molecular sciences
The global rise in type 2 diabetes results from a combination of genetic predisposition with environmental assaults that negatively affect insulin action in peripheral tissues and impair pancreatic β-cell function and survival. Nongenetic heritability of metabolic traits may be an important contributor to the diabetes epidemic. Transfer RNAs (tRNAs...
Knapp, David J H F Fulga, Tudor A
Published in
Methods in molecular biology (Clifton, N.J.)
Transfer RNA (tRNA) and their associated production and processing machinery can be coopted as a versatile tool for the production of guide RNAs (gRNAs) for Cas9-based genome engineering. Using different tRNA variants enables the production of gRNAs at a variety of steady state levels. Furthermore, engineered tRNAs can be used to process gRNAs from...
Mullen, Patrick Abbott, Jamie A Wellman, Theresa Aktar, Mahafuza Fjeld, Christian Demeler, Borries Ebert, Alicia M Francklyn, Christopher S
Published in
The FEBS journal
Charcot-Marie-Tooth disease (CMT) encompasses a set of genetically and clinically heterogeneous neuropathies characterized by length-dependent dysfunction of the peripheral nervous system. Mutations in over 80 diverse genes are associated with CMT, and aminoacyl-tRNA synthetases (ARS) constitute a large gene family implicated in the disease. Despit...
Nesterov-Mueller, Alexander Popov, Roman Seligmann, Hervé
Published in
Life
We propose combinatorial fusion rules that describe the codon assignment in the standard genetic code simply and uniformly for all canonical amino acids. These rules become obvious if the origin of the standard genetic code is considered as a result of a fusion of four protocodes: Two dominant AU and GC protocodes and two recessive AU and GC protoc...
Uršič, Katja
