Ryskalin, Larisa Biagioni, Francesca Busceti, Carla L Giambelluca, Maria A Morelli, Luca Frati, Alessandro Fornai, Francesco
Published in
Cancers
Cellular prion protein (PrPC) is seminal to modulate a variety of baseline cell functions to grant homeostasis. The classic role of such a protein was defined as a chaperone-like molecule being able to rescue cell survival. Nonetheless, PrPC also represents the precursor of the deleterious misfolded variant known as scrapie prion protein (PrPSc). T...
Li, Fang Xu, Yangfan Xu, Xiaofang Ge, Shengfang Zhang, Feifei Zhang, He Fan, Xianqun
Published in
Molecular therapy. Nucleic acids
In cancer cells, a gain of stemness may have profound implications for tumor initiation, aggressiveness, and clinical outcome. However, the molecular mechanisms underlying the self-renewal maintenance of cancer stem-like cells (CSCs) remain elusive. Here, based on analysis of transcriptome sequencing, we identified a long noncoding RNA (lncRNA) nam...
Mullin, Nicholas P Varghese, Joby Colby, Douglas Richardson, Julia M Findlay, Greg M Chambers, Ian
Published in
FEBS letters
The self-renewal efficiency of mouse embryonic stem cells (ESCs) is determined by the concentration of the transcription factor NANOG. While NANOG binds thousands of sites in chromatin, the regulatory systems that control DNA binding are poorly characterised. Here, we show that NANOG is phosphorylated by casein kinase I, and identify target residue...
Yang, Fan Whelan, Eoin C Guan, Xuebing Deng, Bingquan Wang, Shu Sun, Jiachen Avarbock, Mary R Wu, Xin Brinster, Ralph L
Published in
Cell proliferation
Fibroblast growth factor 9 (FGF9) is expressed by somatic cells in the seminiferous tubules, yet little information exists about its role in regulating spermatogonial stem cells (SSCs). Fgf9 overexpression lentivirus was injected into mouse testes, and PLZF immunostaining was performed to investigate the effect of FGF9 on spermatogonia in vivo. Eff...
Zhou, Hengxing Yang, Huan Lu, Lu Li, Xueying Pan, Bin Fu, Zheng Chu, Tianci Liu, Jun Kang, Yi Liu, Lu
...
Published in
Experimental and Therapeutic Medicine
Neural stem cells (NSCs) are characterized by their potential for self-renewal and ability to differentiate into neurons, astrocytes, and oligodendrocytes. They are of great value to scientific studies and clinical applications. Culturing NSCs in vitro is important for characterizing their properties under controlled environmental conditions that m...
Wang, Min Chen, Xia Wu, Yibo Zheng, Qianwen Chen, Wanyin Yan, Yidan Luan, Xiaojin Shen, Cong Fang, Jie Zheng, Bo
...
Published in
Cell proliferation
Stem cell niche regulated the renewal and differentiation of germline stem cells (GSCs) in Drosophila. Previously, we and others identified a series of genes encoding ribosomal proteins that may contribute to the self-renewal and differentiation of GSCs. However, the mechanisms that maintain and differentiate GSCs in their niches were not well unde...
Aulicino, Francesco Pedone, Elisa Sottile, Francesco Lluis, Frederic Marucci, Lucia Cosma, Maria Pia
Published in
Stem cell reports
The Wnt/β-catenin signaling pathway is a key regulator of embryonic stem cell (ESC) self-renewal and differentiation. Constitutive activation of this pathway has been shown to increase mouse ESC (mESC) self-renewal and pluripotency gene expression. In this study, we generated a novel β-catenin knockout model in mESCs to delete putatively functional...
Thankamony, Archana P. Murali, Reshma Karthikeyan, Nitheesh Varghese, Binitha Anu Teo, Wee S. McFarland, Andrea Roden, Daniel L. Holliday, Holly Konrad, Christina Valbirk Cazet, Aurelie
...
Published in
Biomolecules
The basic helix-loop-helix (bHLH) transcription factors inhibitor of differentiation 1 ( Id1 ) and inhibitor of differentiation 3 (Id3) (referred to as Id ) have an important role in maintaining the cancer stem cell (CSC) phenotype in the triple-negative breast cancer (TNBC) subtype. In this study, we aimed to understand the molecular mechanism und...
Salik, Basit Yi, Hangyu Hassan, Nunki Santiappillai, Nancy Vick, Binje Connerty, Patrick Duly, Alastair Trahair, Toby Woo, Andrew J Beck, Dominik
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Published in
Cancer cell
Signals driving aberrant self-renewal in the heterogeneous leukemia stem cell (LSC) pool determine aggressiveness of acute myeloid leukemia (AML). We report that a positive modulator of canonical WNT signaling pathway, RSPO-LGR4, upregulates key self-renewal genes and is essential for LSC self-renewal in a subset of AML. RSPO2/3 serve as stem cell ...
Yu, Fanyuan Wu, Fanzi Li, Feifei Liao, Xueyang Wang, Yitian Li, Xin Wang, Chenglin Shi, Yu Ye, Ling
Published in
Stem cells (Dayton, Ohio)
As a profoundly anabolic regulator of bone, Wnt7b is well acknowledged to enhance osteoblast activities. Here, we report that bone marrow mesenchymal stem cells (BMSCs) are another important population responding to Wnt7b. In this study, we systematically investigated the in vivo role of Wnt7b in BMSCs using transgenic mice, high-throughput RNA-seq...