Published in Cell stem cell
Generation of induced oligodendrocyte progenitor cells (iOPCs) from somatic fibroblasts is a strategy for cell-based therapy of myelin diseases. However, iOPC generation is inefficient, and the resulting iOPCs exhibit limited expansion and differentiation competence. Here we overcome these limitations by transducing an optimized transcription facto...
Published in Frontiers in Oncology
Gastric cancer (GC) is one of the most widespread causes of cancer-related death worldwide. Recently, emerging implied that gastric cancer stem cells (GCSCs) play an important role in the initiation and progression of GC. This subpopulation comprises cells with several features, such as self-renewal capability, high proliferating rate, and ability ...
Published in Cancers
The tumor microenvironment (TME) plays an important role in cell growth, proliferation, migration, immunity, malignant transformation, and apoptosis. Thus, better insight into tumor-host interactions is required. Most of these processes involve the metabolic reprogramming of cells. Here, we focused on this reprogramming in cancerous cells and its e...
Published in Frontiers in Oncology
Tumor progression depends on the collaborative interactions between tumor cells and the surrounding stroma. First-line therapies direct against cancer cells may not reach a satisfactory outcome, such as gastric cancer (GC), with high risk of recurrence and metastasis. Therefore, novel treatments and drugs target the effects of stroma components are...
Published in Stem Cell Reports
In this article, Kime and colleagues show single-cell RNA-seq analysis across key time points within their induced blastocyst-like hemisphere synthetic embryo system. The study reveals how epiblast stem cells cultured in defined media had reprogrammed with zygotic genome activation-related expression and became cells with the critical gene regulati...
Published in Stem Cell Reports
In this article, Napolitano, Rapakoulia, and colleagues present DECCODE, a method for automatic identification of drugs that alone or in combination promote cell conversion and reprogramming. They validate the method for reprogramming of human fibroblasts to induced pluripotent stem cells (hiPSCs), and provide predicted treatments for conversion to...
Published in Frontiers in Cell and Developmental Biology
An expanding repertoire of histone variants and specialized histone chaperone partners showcases the versatility of nucleosome assembly during different cellular processes. Recent research has suggested an integral role of nucleosome assembly pathways in both maintaining cell identity and influencing cell fate decisions during development and norma...
Published in Stem Cell Reports
Chen et al. report a novel approach that produces human microglia (iMG) from induced pluripotent stem cells within 10 days by forced expression of SPI1 and CEBPA together. These iMGs exhibit appropriate physiological functions and can be co-cultured with hiPSC-derived neurons, allowing the study of in vitro neuron-microglia interactions under both ...
Published in Animal biotechnology
Many contrasting reports are available on generation of bovine induced pluripotent stem cells (iPSCs) employing different timelines and culture conditions which signifies reprogramming process varies between species and cell types. The present study determines an optimum time period required to re-initiate reprogramming events in buffalo fibroblast...
Published in Stem Cell Reports
In this article, Okano and colleagues established a species-wide method for deriving transgene-free iPSCs, and discovered primary colony-forming cells showed a neural stem cell-like profile, named induced neural stem cell-like cells (iNSLCs).
