Yang, Guang Zhang, Linfeng Liu, Wenqiang Qiao, Zhibin Shen, Shijun Zhu, Qianshu Gao, Rui Wang, Mengting Wang, Mingzhu Li, Chong
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Published in
Cell stem cell
Differentiated somatic cells can be reprogrammed to totipotent embryos through somatic cell nuclear transfer (SCNT) with low efficiency. The histone deacetylase inhibitor trichostatin A (TSA) has been found to improve SCNT efficiency, but the underlying mechanism remains undetermined. Here, we examined genome-wide H3K9ac during SCNT embryo developm...
Panda, Amitesh Zylicz, Jan J Pasque, Vincent
Published in
Cells
Dosage compensation between the sexes results in one X chromosome being inactivated during female mammalian development. Chromosome-wide transcriptional silencing from the inactive X chromosome (Xi) in mammalian cells is erased in a process termed X-chromosome reactivation (XCR), which has emerged as a paradigm for studying the reversal of chromati...
Pelham-Webb, Bobbie Murphy, Dylan Apostolou, Effie
Published in
Stem cell reports
Higher-order chromatin structure is tightly linked to gene expression and therefore cell identity. In recent years, the chromatin landscape of pluripotent stem cells has become better characterized, and unique features at various architectural levels have been revealed. However, the mechanisms that govern establishment and maintenance of these topo...
Bouma, Marga J Arendzen, Christiaan H Mummery, Christine L Mikkers, Harald Freund, Christian
Published in
Current protocols in stem cell biology
We describe a protocol for efficient generation of human-induced pluripotent stem cells (hiPSCs) from urine-derived cells (UDCs) obtained from adult donors using self-replicative RNA containing the reprogramming factors OCT3/4, SOX2, KLF4, GLIS1, and c-MYC (ReproRNA-OKSGM). After electroporation, transfection efficiency is quantified by measuring O...
Klingenstein, Stefanie Klingenstein, Moritz Kleger, Alexander Liebau, Stefan
Published in
Current protocols in stem cell biology
Keratinocytes, as a primary somatic cell source, offer exceptional advantages compared to fibroblasts, which are commonly used for reprogramming. Keratinocytes can beat fibroblasts in reprogramming efficiency and reprogramming time and, in addition, can be easily and non-invasively harvested from human hair roots. However, there is still much to kn...
Zaret, Kenneth S.
Pioneer transcription factors have the intrinsic biochemical ability to scan partial DNA sequence motifs that are exposed on the surface of a nucleosome and thus access silent genes that are inaccessible to other transcription factors. Pioneer factors subsequently enable other transcription factors, nucleosome remodeling complexes, and histone modi...
Loppin, Benjamin Berger, Frédéric
Nucleosome dynamics and properties are central to all forms of genomic activities. Among the core histones, H3 variants play a pivotal role in modulating nucleosome structure and function. Here, we focus on the impact of H3 variants on various facets of development. The deposition of the replicative H3 variant following DNA replication is essential...
Cai, Chen Yi Meng, Fei Long Rao, Lin Liu, Yun Yue Zhao, Xiao Li
Published in
Yi chuan = Hereditas
自2006年Takahashi和Yamanaka报道生成诱导多能干细胞(induced pluripotent stem cells, iPSCs)以来,多能干细胞领域进入了前所未有的发展状态,在疾病建模、药物发现以及细胞疗法等各方面都发挥重要作用,促进了细胞生物学和再生医学等学科的发展。目前,iPSCs技术已成为研究病理机制的重要工具,利用iPSCs技术筛选的新药物正在研发中,使用iPSCs衍生细胞的临床试验数量也在逐渐增长。iPSCs与基因编辑技术以及3D类器官相结合的最新研究进展促进了iPSCs在疾病研究中的进一步应用。本文介绍了近年来重编程方法的革新,分析了整合病毒载体系统、整合非病毒载体系统、非整合病毒载体系统以及非整合非病毒载体系统四种重编程方法的利弊;同时综述了iPSCs在疾病...
Min, Hyunjung Xu, Li Parrott, Roberta Overall, Christopher C Lillich, Melina Rabjohns, Emily M Rampersad, Rishi R Tarrant, Teresa K Meadows, Norin Fernandez-Castaneda, Anthony
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Published in
Stem cells (Dayton, Ohio)
Mesenchymal stromal cells (MSCs) are widely used in clinical trials because of their ability to modulate inflammation. The success of MSCs has been variable over 25 years, most likely due to an incomplete understanding of their mechanism. After MSCs are injected, they traffic to the lungs and other tissues where they are rapidly cleared. Despite be...
Lee, Byoung-Kwon Seo, Dong-Kwon
Published in
Healthcare (Basel, Switzerland)
Chronic neck pain (CNP) patients have weak deep neck flexors (DNF) and a hyperactive sternocleidomastoid (SCM). The cranio-cervical flexion test (CCFT) promotes activation of the DNF and decreases activity of the SCM, promoting pain recovery, but research suggests SCM activation increases with increasing gaze direction. We aimed to investigate how ...
