Duvergé, Alexis Negroni, Matteo
Delivering transgenes to human cells through transduction with viral vectors constitutes one of the most encouraging approaches in gene therapy. Lentivirus-derived vectors are among the most promising vectors for these approaches. When the genetic modification of the cell must be performed in vivo, efficient specific transduction of the cell target...
Gutierrez-Guerrero, Alejandra Cosset, François-Loïc Verhoeyen, Els
Published in
Viruses
Viruses have been repurposed into tools for gene delivery by transforming them into viral vectors. The most frequently used vectors are lentiviral vectors (LVs), derived from the human immune deficiency virus allowing efficient gene transfer in mammalian cells. They represent one of the safest and most efficient treatments for monogenic diseases af...
Shinohara, Takashi Kanatsu-Shinohara, Mito
Spermatogonial stem cells (SSCs) serve as a resource for producing genetically modified animals. However, genetic manipulation of SSCs has met with limited success. Here, we show efficient gene transfer into SSCs via a lentivirus (FV-LV) using a fusion protein (F), a Sendai virus (SV) envelope protein involved in virion/cell membrane fusion. FV-LVs...
Shinohara, Takashi Kanatsu-Shinohara, Mito
Published in
Stem cell reports
Spermatogonial stem cells (SSCs) serve as a resource for producing genetically modified animals. However, genetic manipulation of SSCs has met with limited success. Here, we show efficient gene transfer into SSCs via a lentivirus (FV-LV) using a fusion protein (F), a Sendai virus (SV) envelope protein involved in virion/cell membrane fusion. FV-LVs...
Radek, Constanze Bernadin, Ornellie Drechsel, Katharina Cordes, Nicole Pfeifer, Rita Sträßer, Pia Mormin, Mirella Gutierrez-Guerrero, Alejandra Cosset, François-loïc Kaiser, Andrew D.
...
Published in
Human Gene Therapy
Cell and gene therapies are finally becoming viable patient treatment options, with both T cell- and hematopoietic stem cell (HSC)-based therapies being approved to market in Europe. However, these therapies, which involve the use of viral vector to modify the target cells, are expensive and there is an urgent need to reduce manufacturing costs. On...
Rosales Gerpe, María C. van Lieshout, Laura P. Domm, Jakob M. Ingrao, Joelle C. Datu, Jodre Walsh, Scott R. Yu, Darrick L. de Jong, Jondavid Krell, Peter J. Wootton, Sarah K.
...
Published in
Viruses
Jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus (ENTV) are small-ruminant betaretroviruses that share high nucleotide and amino acid identity, utilize the same cellular receptor, hyaluronoglucosaminidase 2 (Hyal2) for entry, and transform tissues with their envelope (Env) glycoprotein; yet, they target discrete regions of the resp...
Tomás, Hélio A. Mestre, Daniel A. Rodrigues, Ana F. Guerreiro, Miguel R. Carrondo, Manuel J.T. Coroadinha, Ana Sofia
Published in
Molecular Therapy. Methods & Clinical Development
Lentiviral vectors (LVs) are excellent tools for gene transfer into mammalian cells. It is noteworthy that the first gene therapy treatment using LVs was approved for commercialization in 2017. The G glycoprotein from rhabdovirus vesicular stomatitis virus (VSV-G) is the glycoprotein most used to pseudotype LVs, due to its high efficiency in transd...
Ozog, Stosh Chen, Craig X Simpson, Elizabeth Garijo, Olivia Timberlake, Nina D Minder, Petra Verhoeyen, Els Torbett, Bruce E
Published in
Molecular therapy. Methods & clinical development
Lentiviral vectors (LVs) pseudotyped with the measles virus hemagglutinin (H) and fusion (F) glycoproteins have been reported to more efficiently transduce hematopoietic stem and progenitor cells (HSPCs) compared with vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped LVs. However, a limit to H/F LV use is the low titer of produced vector....
Henss, Lisa Yue, Constanze Kandler, Joshua Faddy, Helen M. Simmons, Graham Panning, Marcus Lewis-Ximenez, Lia Laura Baylis, Sally A. Schnierle, Barbara S.
Published in
Viruses
Background: Alphaviruses are transmitted by arthropod vectors and can be found worldwide. Alphaviruses of the Semliki Forest complex such as chikungunya virus (CHIKV), Mayaro virus (MAYV) or Ross River virus (RRV) cause acute febrile illness and long-lasting arthralgia in humans, which cannot be clinically discriminated from a dengue virus or Zika ...
Kim, Soo-Hyun Lim, Kwang-Il
Published in
Molecules and cells
Retroviral and lentiviral vectors are mostly pseudotyped and often purified and concentrated via ultracentrifugation. In this study, we quantified and compared the stabilities of retroviral [murine leukemia virus (MLV)-based] and lentiviral [human immunodeficiency virus (HIV)-1-based] vectors pseudotyped with relatively mechanically stable envelope...