Wu, Yijian Feng, Sai Sun, Zeao Hu, Yan Jia, Xiao Zeng, Bin
Published in
Frontiers in Bioengineering and Biotechnology
Saccharomyces cerevisiae is one of the most extensively used biosynthetic systems for the production of diverse bioproducts, especially biotherapeutics and recombinant proteins. Because the expression and insertion of foreign genes are always impaired by the endogenous factors of Saccharomyces cerevisiae and nonproductive procedures, various techno...
Huttanus, Herbert M. Triola, Ellin-Kristina H. Velasquez-Guzman, Jeanette C. Shin, Sang-Min Granja-Travez, Rommel S. Singh, Anmoldeep Dale, Taraka Jha, Ramesh K.
Published in
Frontiers in Bioengineering and Biotechnology
Targeted mutagenesis of a promoter or gene is essential for attaining new functions in microbial and protein engineering efforts. In the burgeoning field of synthetic biology, heterologous genes are expressed in new host organisms. Similarly, natural or designed proteins are mutagenized at targeted positions and screened for gain-of-function mutati...
Pan, Xuewei You, Jiajia Tang, Mi Zhang, Xian Xu, Meijuan Yang, Taowei Rao, Zhiming
Published in
Frontiers in Microbiology
Prodigiosin (PG), a red linear tripyrrole pigment produced by Serratia marcescens, has attracted attention due to its immunosuppressive, antimicrobial, and anticancer properties. Although many studies have been used to dissect the biosynthetic pathways and regulatory network of prodigiosin production in S. marcescens, few studies have been focused ...
Liu, Ying Huang, Yuxin Lu, Ran Xin, Fengxue Liu, Guannan
Published in
Trends in biotechnology
Cell fusion is a fundamental biological process that is involved in the development of most eukaryotic organisms. During the fusion process in Saccharomyces cerevisiae, cells respond to pheromones to trigger the MAPK (mitogen-activated protein kinase) cascade to initiate mating, followed by polarization, cell-wall remodeling, membrane fusion, and k...
Yun, Kaiyue Zhang, Yue Li, Shixin Wang, Yan Tu, Ran Liu, Hao Wang, Meng
Published in
Frontiers in Bioengineering and Biotechnology
Erythromycin is a clinically important drug produced by the rare actinomycete Saccharopolyspora erythraea. In the wide-type erythromycin producer S. erythraea NRRL 23338, there is a lack of systematical method for promoter engineering as well as a well-characterized promoter panel for comprehensive metabolic engineering. Here we demonstrated a syst...
Kim, Nancy M. Sinnott, Riley W. Rothschild, Lily N. Sandoval, Nicholas R.
Published in
Frontiers in Bioengineering and Biotechnology
Transcription factor (TF)–promoter pairs have been repurposed from native hosts to provide tools to measure intracellular biochemical production titer and dynamically control gene expression. Most often, native TF–promoter systems require rigorous screening to obtain desirable characteristics optimized for biotechnological applications. High-throug...
Khobragade, Taresh P. Sarak, Sharad Pagar, Amol D. Jeon, Hyunwoo Giri, Pritam Yun, Hyungdon
Published in
Frontiers in Bioengineering and Biotechnology
Herein, we report the development of a multi-enzyme cascade using transaminase (TA), esterase, aldehyde reductase (AHR), and formate dehydrogenase (FDH), using benzylamine as an amino donor to synthesize the industrially important compound sitagliptin intermediate. A panel of 16 TAs was screened using ethyl 3-oxo-4-(2,4,5-trifluorophenyl) butanoate...
Round, James W Robeck, Logan D Eltis, Lindsay D
Published in
ACS synthetic biology
The development of microbial cell factories requires robust synthetic biology tools to reduce design uncertainty and accelerate the design-build-test-learn process. Herein, we developed a suite of integrative genetic tools to facilitate the engineering of Rhodococcus, a genus of bacteria with considerable biocatalytic potential. We first created pR...
Dacquay, Louis C McMillen, David R
Published in
FEMS Yeast Research
The paper presents the design and optimization of a novel yeast biosensor using an engineered promoter to sense oxidative stress, and by extension, the presence of reactive oxygen species.
Tong, Yingjia Zhou, Jingwen Zhang, Liang Xu, Peng
Published in
ACS Synthetic Biology
Violacein is a naturally occurring anticancer therapeutic compound with deep purple color. In this work, we harnessed the modular and combinatorial feature of a Golden Gate assembly method to construct a library of violacein producing strains in the oleaginous yeast Yarrowia lipolytica , where each gene in the violacein pathway was controlled by th...
