Liu, Linlin Yang, Man Wang, Naiqiang Li, Li Chen, Zi-Jiang Zhang, Cong
Published in
Journal of cellular biochemistry
FKBP12, known as FK506 binding protein, binds to immunosuppressive drug FK506, which must be taken by patients who received organ transplant. The side effect of FK506 is that women have difficulties in bearing a baby, so it is important to find the reason of their subfertility. This research explored the expression of FKBP12 in pre-implantation emb...
Parmegiani, Lodovico Tatone, Carla Cognigni, Graciela Estela Bernardi, Silvia Troilo, Enzo Arnone, Alessandra Maccarini, Antonio Manuel Di Emidio, Giovanna Vitti, Maurizio Filicori, Marco
...
Published in
Reproductive BioMedicine Online
Nowadays, human oocytes/embryos are cryopreserved via slow freezing or vitrification. The aim of this study was to evaluate a rapid warming protocol for slow-frozen human oocytes based on the standard warming procedure for vitrification. This was a prospective study on 216 sibling oocytes randomized for either conventional rapid thawing or rapid wa...
Wakai, Takuya Ito, Junya Fissore, Rafael A.
Published in
Principles of Cloning
Since the birth of the first cloned sheep, “Dolly”, many studies have reported the successful use of cloning by somatic cell nuclear transfer in other mammalian species. In spite of these successes, the efficiency of this technique is low, with less than 10% of generated embryos resulting in offspring. Factors of genetic, epigenetic, and technical ...
Dieci, Cecilia Lodde, Valentina Franciosi, Federica Lagutina, Irina Tessaro, Irene Modina, Silvia C Albertini, David F Lazzari, Giovanna Galli, Cesare Luciano, Alberto M
...
Published in
Biology of reproduction
In the pig, the efficiency of in vitro embryo production and somatic cell nuclear transfer (SCNT) procedures remains limited. It has been suggested that prematuration treatments (pre-IVM) based on the prolongation of a patent, bidirectional crosstalk between the oocyte and the cumulus cells through gap junction mediate communication (GJC), with the...
Ma, YuFang Li, Yan Wei, HengXi Li, QiuYan Fang, Rui Zhao, Rui Zhang, Kun Xue, Kai Lou, YanKun Dai, YunPing
...
Published in
Science in China Series C: Life Sciences
The effects of chemical activation on birth efficiency of cloned pigs were studied by investigating the developmental process from porcine oocyte activation to birth of cloned pigs. Three different activation methods were used: (i) Electroporation (Ele); (ii) Ele followed by incubation with 6-dimethylaminopurine (6-DMAP); and (iii) Ele followed by ...
Santos, T. Almeida Dias, C. Henriques, P. Brito, R. Barbosa, A. Regateiro, F. Santos, A. Almeida
Published in
Journal of Assisted Reproduction and Genetics
Purpose: Spontaneous parthenogenetically activated noninseminated oocytes and failed fertilized oocytes after ART activated by puromycin were studied to assess cleavage ability and the cytogenetic constitution of the resulting embryos. Methods: Failed fertilized oocytes were exposed to puromycin, and whenever activation occurred, they were further ...
