Nagle, Peter W Coppes, Robert P
Published in
Cells
The majority of cancer patients will be treated with radiotherapy, either alone or together with chemotherapy and/or surgery. Optimising the balance between tumour control and the probability of normal tissue side effects is the primary goal of radiation treatment. Therefore, it is imperative to understand the effects that irradiation will have on ...
Döpper, Hannah Menges, Julia Bozet, Morgane Brenzel, Alexandra Lohmann, Dietmar Steenpass, Laura Kanber, Deniz
Published in
Current protocols in stem cell biology
Structures resembling whole organs, called organoids, are generated using pluripotent stem cells and 3D culturing methods. This relies on the ability of cells to self-reorganize after dissociation. In combination with certain supplemented factors, differentiation can be directed toward the formation of several organ-like structures. Here, a protoco...
Patterson, Logan Allen, Jawara Posey, Isabella Shaw, Jeremy Joseph Porter Costa-Pinheiro, Pedro Walker, Susan J Gademsey, Alexis Wu, Xinqun Wu, Shaoguang Zachos, Nicholas C
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Enterotoxigenic Bacteroides fragilis (ETBF) is a commensal bacterium of great importance to human health due to its ability to induce colitis and cause colon tumor formation in mice through the production of B. fragilis toxin (BFT). The formation of tumors is dependent on a pro-inflammatory signaling cascade, which begins with the disruption of epi...
Velazquez, Jeremy J LeGraw, Ryan Moghadam, Farzaneh Tan, Yuqi Kilbourne, Jacquelyn Maggiore, Joseph C Hislop, Joshua Liu, Silvia Cats, Davy Chuva de Sousa Lopes, Susana M
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Published in
Cell systems
Pluripotent stem cell (PSC)-derived organoids have emerged as novel multicellular models of human tissue development but display immature phenotypes, aberrant tissue fates, and a limited subset of cells. Here, we demonstrate that integrated analysis and engineering of gene regulatory networks (GRNs) in PSC-derived multilineage human liver organoids...
Yim, Sung-Kun Kim, Kian Chun, SangHo Oh, TaeHawn Jung, WooHuk Jung, KyooJin Yun, Chul-Ho
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Marine drugs
Phenolic compounds and carotenoids are potential inhibitors of cytochrome P450s. Sixteen known compounds, phenolic compounds and carotenoids from seaweed were examined for potential inhibitory capacity against CYP1A2 and CYP3A4 in silico and in vitro. Morin, quercetin, and fucoxanthin inhibited the enzyme activity of CYP1A2 and CYP3A4 in a dose-dep...
Alvarado, Juan C Fuentes-Santamaría, Verónica Melgar-Rojas, Pedro Gabaldón-Ull, María C Cabanes-Sanchis, José J Juiz, José M
Published in
Antioxidants (Basel, Switzerland)
Noise induces oxidative stress in the cochlea followed by sensory cell death and hearing loss. The proof of principle that injections of antioxidant vitamins and Mg2+ prevent noise-induced hearing loss (NIHL) has been established. However, effectiveness of oral administration remains controversial and otoprotection mechanisms are unclear. Using aud...
Parvatam, Surat Bharadwaj, Sham Radha, Vegesna Rao, Madhusudhana
Published in
Journal of Biosciences
The low translational efficiency of animal models to humans, and the development of new-age methodologies that are human-cell based, is fuelling a paradigm change across the globe. In this perspectives paper, we discuss the current state of research, funding, and regulation in these 21st century technologies, including organoids and organ-on-chip i...
Kang, Sung-Min Kim, Daehan Lee, Ji-Hoon Takayama, Shuichi Park, Joong Yull
Published in
Advanced healthcare materials
3D in vitro model systems such as spheroids and organoids provide an opportunity to extend the physiological understanding using recapitulated tissues that mimic physiological characteristics of in vivo microenvironments. Unlike 2D systems, 3D in vitro systems can bridge the gap between inadequate 2D cultures and the in vivo environments, providing...
Engevik, Melinda A Danhof, Heather A Shrestha, Ritu Chang-Graham, Alexandra L Hyser, Joseph M Haag, Anthony M Mohammad, Mahmoud A Britton, Robert A Versalovic, James Sorg, Joseph A
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Gut microbes
Antibiotic resistance is one of the world's greatest public health challenges and adjunct probiotic therapies are strategies that could lessen this burden. Clostridioides difficile infection (CDI) is a prime example where adjunct probiotic therapies could decrease disease incidence through prevention. Human-derived Lactobacillus reuteri is a probio...
Katsura, Hiroaki Sontake, Vishwaraj Tata, Aleksandra Kobayashi, Yoshihiko Edwards, Caitlin E. Heaton, Brook E. Konkimalla, Arvind Asakura, Takanori Mikami, Yu Fritch, Ethan J.
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Published in
Cell Stem Cell
Tata and colleagues report defined conditions for long-term expansion and differentiation of adult human primary alveolar stem cells. Cultured AT2s are conducive to SARS-CoV-2 infection and elicit transcriptome-wide changes that mirror COVID-19 histopathology, including upregulation of inflammatory responses, cell death, and downregulation of surfa...