Chen, Z Xie, H Yuan, J Lan, Y Xie, Z
Published in
International endodontic journal
To investigate the potential role of Krüppel-like factor 6 (KLF6) in the odontoblastic differentiation of immortalized dental papilla mesenchymal cells (iMDP-3) cells. Alizarin Red S (ARS) and Alkaline phosphatase (ALP) staining was used to examine the mineralization effect of iMDP-3 cells after odontoblastic induction. Real-time PCR and western bl...
Lee, Yoon Seon Park, Yeoung-Hyun Lee, Dong-Seol Seo, You-Mi Lee, Ji-Hyun Park, Joo-Hwang Choung, Han-Wool Park, So-Hyun Shon, Won Jun Park, Joo-Cheol
...
Published in
Materials
We aim to examine the effects of a newly developed peptide derived from CPNE7 (Cpne7-DP) in tertiary dentin formation and peritubular space occlusion, and comprehensively evaluate its potential as a bioactive therapeutic agent. Human dental pulp cells (HDPCs) and a mouse pre-odontoblast cell line, MDPC-23, were chosen for in vitro studies to charac...
Lee, Eun-Cheol Kim, Yu-Mi Lim, Han-Moi Ki, Ga-Eun Seo, Young-Kwon
Published in
International Journal of Molecular Sciences
The role of dental pulp stem cells (DPSCs) in dental tissue regeneration is gaining attention because DPSCs can differentiate into odontoblasts and other specialized cell types. Epigenetic modification has been found to play an important role in cell differentiation and regulation, among which histone deacetylase (HDAC) is involved in suppressing g...
Odlén, Karin Fält, Felicia Dahl, Sara Aidoukovitch, Alexandra Ericson, Dan Nilsson, Bengt-Olof Hedenbjörk-Lager, Anders
Published in
Acta odontologica Scandinavica
Objective: Odontoblasts are thought to be involved in innate immunity but their precise role in this process is not fully understood. Here, we assess effects of lipopolysaccharide (LPS) and lipoteichoic acid (LTA), produced by Gram-negative and Gram-positive bacteria, respectively, on matrix metalloproteinase-8 (MMP-8), interleukin-6 (IL-6) and cat...
Driesen, Ronald B. Hilkens, Petra Smisdom, Nick Vangansewinkel, Tim Dillen, Yörg Ratajczak, Jessica Wolfs, Esther Gervois, Pascal Ameloot, Marcel Bronckaers, Annelies
...
Published in
Frontiers in Cell and Developmental Biology
Fibroblast activation protein-α (FAPα) is a membrane protein with dipeptidyl-peptidase and type I collagenase activity and is expressed during fetal growth. At the age of adolescence, FAPα expression is greatly reduced, only emerging in pathologies associated with extracellular matrix remodeling. We determined whether FAPα is expressed in human den...
Shi, C Yuan, Y Guo, Y Jing, J Ho, T V Han, X Li, J Feng, J Chai, Y
Published in
Journal of dental research
Bone morphogenetic protein (BMP) signaling performs multiple essential functions during craniofacial development. In this study, we used the adult mouse incisor as a model to uncover how BMP signaling maintains tissue homeostasis and regulates mesenchymal stem cell (MSC) fate by mediating WNT and FGF signaling. We observed a severe defect in the pr...
Xiong, Y Fang, Y Qian, Y Liu, Y Yang, X Huang, H Huang, H Li, Y Zhang, X Zhang, Z
...
Published in
Journal of dental research
The Wnt ligands display varied spatiotemporal expression in the epithelium and mesenchyme in the developing tooth. Thus far, the actions of these differentially expressed Wnt ligands on tooth development are not clear. Shh expression specifies the odontogenic epithelium during initiation and is consistently restricted to the dental epithelium durin...
Paula, Anabela Laranjo, Mafalda Marto, Carlos Miguel Abrantes, Ana Margarida Casalta-Lopes, João Gonçalves, Ana Cristina Sarmento-Ribeiro, Ana Bela Ferreira, Manuel M. Botelho, Maria Filomena Carrilho, Eunice
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Published in
Materials
(1) Background: When pulp exposure occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain the vitality and function of the tissue. The aim of this work was to assess the cytotoxicity and bioactivity of three different direct pulp capping materials, calcium hydroxide (Life®), mineral trioxide aggregate (WhiteProRoot®MTA)...
Xie, Furong Dai, Qinggang Liu, Xiao Wang, Jun
Published in
Frontiers in Physiology
mTORC1 signaling plays an important role in extracellular and intracellular signals, including growth factors, nutrients, energy metabolism, and stress. However, the functional role of mTORC1 in dentinogenesis is unknown. To study the role of Raptor/mTORC1 in dentinogenesis, an Raptorfl/fl; Osx-Cre (Rap-Osx) mouse, in which Raptor was conditionally...
Wu, Qi Li, Jing Song, Pingping Chen, Jing Xu, Yuanzhi Qi, Shengcai Ma, Ji Pan, Qiuhui
Published in
Connective tissue research
Neurotrophin receptor-interacting MAGE homologue (Nrage) plays an important role in bone development and the metabolism of normal skeletal structures. Our previous study showed that Nrage inhibited the odontogenic differentiation of mouse dental pulp cells. However, the potential roles and mechanism of Nrage in regulating odontogenic differentiatio...
