Hu, Yaru Zhu, Le Ma, Cong
Published in
Frontiers in Cell and Developmental Biology
Formation of the trans -SNARE complex is believed to generate a force transfer to the membranes to promote membrane fusion, but the underlying mechanism remains elusive. In this study, we show that helix-breaking and/or length-increasing insertions in the juxtamembrane linker region of synaptobrevin-2 exert diverse effects on liposome fusion, in a ...
Guček, Alenka
Muggia, Lucia Ametrano, Claudio G Sterflinger, Katja Tesei, Donatella
Published in
Life (Basel, Switzerland)
Fungi are among the most successful eukaryotes on Earth: they have evolved strategies to survive in the most diverse environments and stressful conditions and have been selected and exploited for multiple aims by humans. The characteristic features intrinsic of Fungi have required evolutionary changes and adaptations at deep molecular levels. Omics...
Wang, Lin Xiang, Ye
Published in
Viruses
Severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 are enveloped, positive-sense, single-stranded RNA viruses and causes of epidemic diseases that have resulted in public health emergencies worldwide. Angiotensin-converting enzyme 2 (ACE2) is the receptor that allows the entry of these two viruses into host cells, a key step in...
Francés-Monerris, Antonio Hognon, Cécilia Miclot, Tom García-Iriepa, Cristina Iriepa, Isabel Terenzi, Alessio Grandemange, Stéphanie Barone, Giampaolo Marazzi, Marco Monari, Antonio
...
Published in
Journal of proteome research
The emergence in late 2019 of the coronavirus SARS-CoV-2 has resulted in the breakthrough of the COVID-19 pandemic that is presently affecting a growing number of countries. The development of the pandemic has also prompted an unprecedented effort of the scientific community to understand the molecular bases of the virus infection and to propose ra...
André, Timon Classen, Jessica Brenner, Philipp Betts, Matthew J. Dörr, Bernhard Kreye, Susanne Zuidinga, Birte Meijer, Marieke Russell, Robert B. Verhage, Matthijs
...
Published in
eNeuro
Sec1/Munc18 proteins play a key role in initiating the assembly of N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, the molecular fusion machinery. Employing comparative structure modeling, site specific crosslinking by single amino acid substitutions with the photoactivatable unnatural amino acid p-Benzoyl-phenylala...
Takikawa, Masato Fujisawa, Mizuki Yoshino, Kazuma Takeoka, Shinji
Published in
International Journal of Nanomedicine
Aim The uptake pathway of liposomes into cells is mainly via endocytosis or membrane fusion; however, the relationship between the uptake pathway and the intracellular pharmacokinetics of the liposome components remains unclear. This study aimed at revealing the relationship by using cationic liposomes having similar physical properties and differe...
Zhao, Xiaoli Tian, Jenny Jingxin Yu, Hua Bryksa, Brian C Dupuis, John H Ou, Xiuyuan Qian, Zhaohui Song, Chen Wang, Shenlin Yada, Rickey Y
...
Published in
The Journal of biological chemistry
In plants, many natural defense mechanisms include cellular membrane fusion as a way to resist infection by external pathogens. Several plant proteins mediate membrane fusion, but the detailed mechanism by which they promote fusion is less clear. Understanding this process could provide valuable insights into these proteins' physiological functions...
Gu, Xiaoyan Brennan, Adrian Wei, Wenbin Guo, Guangqin Lindsey, Keith
Published in
Evolutionary Bioinformatics Online
Communication systems within and between plant cells involve the transfer of ions and molecules between compartments, and are essential for development and responses to biotic and abiotic stresses. This in turn requires the regulated movement and fusion of membrane systems with their associated cargo. Recent advances in genomics has provided new re...
Tahir, Mukarram A. Guven, Zekiye P. Arriaga, Laura R. Tinao, Berta Yang, Yu-Sang Sabrina Bekdemir, Ahmet Martin, Jacob T. Bhanji, Alisha N. Irvine, Darrell Stellacci, Francesco
...
Published in
Proceedings of the National Academy of Sciences of the United States of America
Lipid membrane fusion is an essential process for a number of critical biological functions. The overall process is thermodynamically favorable but faces multiple kinetic barriers along the way. Inspired by nature’s engineered proteins such as SNAP receptor [soluble N-ethylmale-imide-sensitive factor-attachment protein receptor (SNARE)] complexes o...
