Published in Frontiers in Immunology
The monocyte/macrophage lineage has been shown to be involved in the promotion of a protumoral tumor microenvironment and resistance to treatment in B cell lymphomas. However, it is still poorly described at the single cell level, and tissue samples are not easily accessible. Thus, a detailed analysis of the circulating myeloid cell compartment in ...
Published in European journal of immunology
Increased incidence of narcolepsy type 1 (NT1) was observed following Pandemrix®-vaccination, initiated as a preventive measure against the 2009 Influenza pandemic. Here, single cell analysis was conducted to suggest a lower number of CD8+ CD27+ T cells among these patients. These findings provide understanding into the autoimmune pathogenesis of N...
Published in Cell Reports Medicine
Chevrier et al. characterize the innate immune response in mild and severe COVID-19 patients and healthy individuals by mass cytometry and serum proteomics. They demonstrate profound phenotypic changes after SARS-CoV-2 infection and ongoing inflammation, with an innate signature switch in severe COVID-19 patients late in the disease course.
Published in Frontiers in Immunology
Aberrant immune cell infiltrates and microcircumstances represent characteristic features of liver fibrosis. In this study, we profiled the transcriptomes of intrahepatic CD45+ immune cells, from mice, using single-cell RNA sequencing (scRNA-seq) technology to understand the landscape of intrahepatic immune cells during the pathogenesis of fibrosis...
Published in Cancers
The p53 protein and its post-translational modifications are distinctly expressed in various normal cell types and malignant cells and are usually detected by immunohistochemistry and flow cytometry in contemporary diagnostics. Here, we describe an approach for simultaneous multiparameter detection of p53, its post-translational modifications and p...
Published in Frontiers in Immunology
Vi-polysaccharide conjugate vaccines are efficacious against cases of typhoid fever; however, an absolute correlate of protection is not established. In this study, we investigated the leukocyte response to a Vi-tetanus toxoid conjugate vaccine (Vi-TT) in comparison with a plain polysaccharide vaccine (Vi-PS) in healthy adults subsequently challeng...
Published in Allergy
Since their discovery in the 60s´, B cells have been extensively studied because of their unique and critical role in immunoglobulin (Ig) production. In addition, B cells contribute to other aspects of the immune response via antigen presentation to T cells and cytokine production. Despite the intensive study of B cells, its classification in diffe...
Published in European journal of immunology
CD56+ T cells are a group of pro-inflammatory CD3+ lymphocytes with characteristics of natural killer cells, being involved in antimicrobial immune defense. Here, we performed deep phenotypic profiling of CD3+ CD56+ cells in peripheral blood of normal human donors and individuals sensitized to birch-pollen or/and house dust mite by high-dimensional...
Published in bioRxiv
For an emerging disease like COVID-19, systems immunology tools may quickly identify and quantitatively characterize cells associated with disease progression or clinical response. With repeated sampling, immune monitoring creates a real-time portrait of the cells reacting to a novel virus before disease specific knowledge and tools are established...
Published in Angewandte Chemie (International ed. in English)
Mass cytometry is attracting significant attention for its spatiotemporal high-throughput single-cell analysis. As the first demonstration of simultaneous detection of single-cell proteins and untargeted metabolites, a multi-dimensional organic mass cytometry system was established by a simple microfluidic chip connected to a nanoelectrospray mass ...
